Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Respiratory syncytial virus IgG ELISA Kit (RSV)
Intra-assay Sample n Mean SD CV% Pos.Serum 24 8.5% Inter-assay Sample n Mean SD CV% Pos.Serum 12 6%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Respiratory syncytial virus (RSV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Respiratory syncytial virus in Human serum and plasma.
A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgG sample captured in plate.
The antigen preparation (inactivated) was virus and viral components from Vero cell culture purified by centrifugation.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Anti-Respiratory syncytial virus (IgG) Coated Microplate (12 x 8 wells) 1 unit Cover foil 1 unit IgG Sample Diluent yellow buffer, white cap 1 x 100ml Respiratory syncytial virus anti-IgG HRP Conjugate blue solution, black cap 1 x 20ml Respiratory syncytial virus IgG Cut-off Control yellow solution, green cap 1 x 3ml Respiratory syncytial virus IgG Negative Control yellow solution, blue cap 1 x 2ml Respiratory syncytial virus IgG Positive Control yellow solution, red cap 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
- RNA Virus
- single stranded RNA negative strand virus
- Resp. Syncytial Virus
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab108765 has been referenced in 2 publications.
- Ezzat K et al. The viral protein corona directs viral pathogenesis and amyloid aggregation. Nat Commun 10:2331 (2019). PubMed: 31133680
- Grandin C et al. Evidence for an intranasal immune response to human respiratory syncytial virus infection in cynomolgus macaques. J Gen Virol 96:782-92 (2015). PubMed: 25537374