Overview

  • Product name

    Human Retinol binding protein ELISA Kit (RBP)
    See all Retinol binding protein kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.008 µg/ml
  • Range

    0.008 µg/ml - 0.5 µg/ml
  • Recovery

    98 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Retinol binding protein (RBP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein concentrations in Human milk, urine, CSF, saliva and cell culture supernatants.


    A Retinol binding protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • RBP measured in various samples showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab108899.

Protocols

References

ab108899 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us and your interest in our products.

The reference range for retinol binding protein in urine sample using the Retinol binding protein Human ELISA Kit (ab108899) has not been determined. The kit has been used with urine samples from donors with no known diseases or conditions that may affect the concentrations. The readings from this fell within the range of 0.08 – 1.04 ug/ml. I hope this information has been of help.

The products that Abcam provide are intended for research and development use only. They are not intended for use in diagnostic or therapeutic applications. I'd like to draw your attention to our terms and conditions:

https://www.abcam.com/index.html?pageconfig=terms

If I can be of any further assistance, please do not hesitate to let me know.

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Answer

Thank you for contacting us and sorry for the delay in getting back to you in regards to the Retinol binding protein Human ELISA Kit (ab108899).

I now have confirmation from the source of this kit that with urine samples, you should not need to centrifuge the samples prior to freezing. The samples taken can be frozen, then when they are ready to perform the assay, defrosted, centrifuged then assayed.

I hope this information answers your question.

I spoke to my colleagues in marketing in regards to the quote for 9-12 kits. We were wondering if you had used the kit yet? If you have not tried it as yet, we would recommend that you just purchase one to start off with to see if it would be suitable for the experiments you hope to perform, then place an order for the rest of the kits.

Have you been able to have a look at the NGAL kits I sent you? Do any of them seem suitable?

https://www.abcam.com/index.html?pageconfig=searchresults&pt=4&search=ngal


I look forward to receiving your reply.

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Answer

Thank you for your reply.

I am in contact with our marketing team and will get back to you with a quote as soon as possible. I will also let you know as soon as I have confirmation of what the best way of preserving the urine samples is.

I look forward to hearing which NGAL kits you would be interested in.

If you have any further questions, please do not hesitate to let me know.

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Answer

Thank you for contacting us yesterday. As discussed over the phone, I am currently seeking further information as to the best method of preparing your urine samples to be used with the Retinol binding protein Human ELISA Kit (ab108899). I will get back to you with this information as soon as I can.

I am sorry but my previous email I sent to you in regards to the other kits you are interested did not get delivered to the correct email address. I am sorry for this. Here is the email I sent:

Following our conversation over the phone, I have had a look through our catalogue and it seems we do have a number of assays developed against NGAL. These can be accessed from the following link:

https://www.abcam.com/index.html?pageconfig=searchresults&pt=4&search=ngal

If you would like to have a look through them and see which you would be most interested in I can generate a quote for you (for9-12 kits)forthisas well as the Retinol binding protein Human ELISA Kit (ab108899).

I look forward to receiving your reply

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Answer

Thank you for contacting us.

The total cost for 1 kits (ab108899) is GBP363 including the packaging and delivery charges.

1 kit will be suitable for 96 reactions in 96 well plate.

Unopened kit is guaranteed for 6 months if stored as recommended on the datasheet. Please check the protocol for individual component storing temperature.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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