Human Retinol binding protein ELISA Kit (RBP) (ab137991)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Human Retinol binding protein ELISA Kit (RBP) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Intra-assay 5.1% Inter-assay Sample n Mean SD CV% Inter-assay 10.1% -
Sample type
Serum, Plasma, Cell Lysate -
Assay type
Competitive -
Sensitivity
0.17 µg/ml -
Range
0.195 µg/ml - 50 µg/ml -
Recovery
98 %
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Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Retinol Binding Protein (RBP) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RBP in plasma and serum.
A Retinol Binding Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Retinol Binding Protein is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Retinol Binding Protein captured in plate.
The immunogen for the RBP-capture antibody is total RBP and thus the capture antibody should detect both RBP1 and RPB4, among others.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml Biotinylated Retinol Binding Protein (Lyophilized) 1 vial Chromogen Substrate 1 x 8ml Retinol Binding Protein Microplate (12 x 8 well strips) 1 unit Retinol Binding Protein Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Intracellular transport of retinol. -
Tissue specificity
Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Cellular retinol-binding protein
- Cellular retinol-binding protein I
- CRBP
see all -
Database links
- Entrez Gene: 5947 Human
- SwissProt: P09455 Human
- Unigene: 529571 Human
Images
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Typical Standard CurveRepresentative Standard Curve using ab137991.
Protocols
References
This product has been referenced in:
- Wu J et al. Urinary RBP and NGAL Levels are Associated with Nephropathy in Patients with Type 2 Diabetes. Cell Physiol Biochem 42:594-602 (2017). Read more (PubMed: 28954270) »
