Overview

  • Product name

    Human Retinol binding protein ELISA Kit (RBP)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Intra-assay 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Inter-assay 10.1%
  • Sample type

    Serum, Plasma, Cell Lysate
  • Assay type

    Competitive
  • Sensitivity

    0.17 µg/ml
  • Range

    0.195 µg/ml - 50 µg/ml
  • Recovery

    98 %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Retinol Binding Protein (RBP) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RBP in plasma and serum.


    A Retinol Binding Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Retinol Binding Protein is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Retinol Binding Protein captured in plate.


    The immunogen for the RBP-capture antibody is total RBP and thus the capture antibody should detect both RBP1 and RPB4, among others.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 1 x 30ml
    Biotinylated Retinol Binding Protein (Lyophilized) 1 vial
    Chromogen Substrate 1 x 8ml
    Retinol Binding Protein Microplate (12 x 8 well strips) 1 unit
    Retinol Binding Protein Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Intracellular transport of retinol.
  • Tissue specificity

    Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.
  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Alternative names

    • Cellular retinol-binding protein
    • Cellular retinol-binding protein I
    • CRBP
    • CRBP-I
    • RBP1
    • RET1_HUMAN
    • Retinol-binding protein 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab137991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab137991.

Protocols

References

ab137991 has not yet been referenced specifically in any publications.

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