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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Retinoic & Retinoid
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Human Retinol binding protein ELISA Kit (RBP) (ab137991)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.17 µg/ml
    • Range: 0.195 µg/ml - 50 µg/ml
    • Sample type: Cell Lysate, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Competitive
    • Reacts with: Human

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    Recombinant Human RBP1 protein (ab115723)

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    Overview

    • Product name

      Human Retinol binding protein ELISA Kit (RBP)
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Intra-assay 5.1%
      Inter-assay
      Sample n Mean SD CV%
      Inter-assay 10.1%
    • Sample type

      Serum, Plasma, Cell Lysate
    • Assay type

      Competitive
    • Sensitivity

      0.17 µg/ml
    • Range

      0.195 µg/ml - 50 µg/ml
    • Recovery

      98 %

    • Assay time

      3h 00m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Retinol Binding Protein (RBP) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RBP in plasma and serum.


      A Retinol Binding Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Retinol Binding Protein is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Retinol Binding Protein captured in plate.


      The immunogen for the RBP-capture antibody is total RBP and thus the capture antibody should detect both RBP1 and RPB4, among others.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent N Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 1 x 30ml
      Biotinylated Retinol Binding Protein (Lyophilized) 1 vial
      Chromogen Substrate 1 x 8ml
      Retinol Binding Protein Microplate (12 x 8 well strips) 1 unit
      Retinol Binding Protein Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 12ml
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • Nuclear Hormone Receptors
      • Retinoic & Retinoid
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Retinoic & Retinoid
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Signal transduction proteins ELISA kits
    • Function

      Intracellular transport of retinol.
    • Tissue specificity

      Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.
    • Sequence similarities

      Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
    • Domain

      Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P09455 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Cellular retinol-binding protein
      • Cellular retinol-binding protein I
      • CRBP
      • CRBP-I
      • CRBP1
      • CRBP2
      • RBP1
      • RBP2
      • RBP4
      • RBPC
      • RET1_HUMAN
      • Retinol binding protein 1
      • Retinol binding protein 1 cellular
      • Retinol binding protein 2 cellular
      • Retinol binding protein 4 plasma
      • Retinol-binding protein 1
      see all
    • Database links

      • Entrez Gene: 5948 Human
      • Entrez Gene: 5950 Human
      • Entrez Gene: 5947 Human
      • Omim: 180260 Human
      • SwissProt: P09455 Human
      • SwissProt: P02753 Human
      • SwissProt: P50120 Human
      • Unigene: 529571 Human

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative Standard Curve using ab137991.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • SDS download

        • Datasheet download

          Download

        References (1)

        Publishing research using ab137991? Please let us know so that we can cite the reference in this datasheet.

        ab137991 has been referenced in 1 publication.

        • Wu J  et al. Urinary RBP and NGAL Levels are Associated with Nephropathy in Patients with Type 2 Diabetes. Cell Physiol Biochem 42:594-602 (2017). PubMed: 28954270

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