Key features and details
- Suitable for: Blocking
Product nameHuman RHEB peptide
See all RHEB proteins and peptides
Our Abpromise guarantee covers the use of ab194764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-RHEB antibody [EPR2971] (ab92313)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C long term.
- Ras homolog enriched in brain 2, formerly
- GTP binding protein Rheb
- GTP-binding protein Rheb
FunctionStimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
Tissue specificityUbiquitous. Highest levels observed in skeletal and cardiac muscle.
Sequence similaritiesBelongs to the small GTPase superfamily. Rheb family.
modificationsFarnesylation is important for efficiently activating mTORC1-mediated signaling.
Cellular localizationCell membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab194764 has been referenced in 1 publication.
- Agarwal S et al. p53 Deletion or Hotspot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb. Mol Cancer Res 14:66-77 (2016). Blocking ; Human . PubMed: 26385560