Key features and details
- Rabbit polyclonal to Human Rhinovirus 3C protease
- Suitable for: WB, IP
- Isotype: IgG
Product nameAnti-Human Rhinovirus 3C protease antibody
DescriptionRabbit polyclonal to Human Rhinovirus 3C protease
Specificityab183574 recognizes the cleavage site of Human rhinovirus 3C protease. Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Other species
Synthetic peptide corresponding to Human Rhinovirus 3C protease. Synthetic peptide recognizing the cleavage site of Human Rhinovirus 3C protease.
Database link: Q82081
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.01% BSA, 30% Glycerol, 69% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000.|
|IP||Use at an assay dependent concentration.
Use 4 µg.
RelevanceHuman rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage.
- SwissProt: Q82081 Other species
All lanes : Anti-Human Rhinovirus 3C protease antibody (ab183574) at 1/1000 dilution
Lane 1 : Uncleaved human rhinovirus 3C protease
Lane 2 : Cleaved human rhinovirus 3C protease
Lysates/proteins at 1 µg per lane.
All lanes : Goat anti-rabbit IgG-HRP at 1/15000 dilution
Immunoprecipitation of Human Rhinovirus 3C protease was performed on a control protein containing GST and the HRV3c cleavage site. Antigen-antibody complexes were formed by incubating 100ug HeLa lysate containing 2ug control protein with 4ug of ab183574 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul of Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Both the lysate and the immune complexes were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a GST monoclonal antibody at a dilution of 1:1500 overnight rotating at 4°C, washed in TBST, and probed with an IP detection reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed. Note: the IP fraction resulted in proteolytic activity of the GST-control protein.
ab183574 has not yet been referenced specifically in any publications.