Overview

  • Product name

    Anti-Human Rhinovirus 3C protease antibody
  • Description

    Rabbit polyclonal to Human Rhinovirus 3C protease
  • Host species

    Rabbit
  • Specificity

    ab183574 recognizes the cleavage site of Human rhinovirus 3C protease. Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Synthetic peptide corresponding to Human Rhinovirus 3C protease. Synthetic peptide recognizing the cleavage site of Human Rhinovirus 3C protease.
    Database link: Q82081

Properties

Applications

Our Abpromise guarantee covers the use of ab183574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000.
IP Use at an assay dependent concentration.

Use 4 µg.

Target

  • Relevance

    Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage.
  • Database links

    Images

    • All lanes : Anti-Human Rhinovirus 3C protease antibody (ab183574) at 1/1000 dilution

      Lane 1 : Uncleaved human rhinovirus 3C protease
      Lane 2 : Cleaved human rhinovirus 3C protease

      Lysates/proteins at 1 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG-HRP at 1/15000 dilution
    • Immunoprecipitation of Human Rhinovirus 3C protease was performed on a control protein containing GST and the HRV3c cleavage site. Antigen-antibody complexes were formed by incubating 100ug HeLa lysate containing 2ug control protein with 4ug of ab183574 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul of Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Both the lysate and the immune complexes were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a GST monoclonal antibody at a dilution of 1:1500 overnight rotating at 4°C, washed in TBST, and probed with an IP detection reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed. Note: the IP fraction resulted in proteolytic activity of the GST-control protein.

    References

    ab183574 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Nasal epithelial cells)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    20 µg
    Treatment
    Rhinovirus
    Specification
    Nasal epithelial cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Dr. Kyeongah Kim

    Verified customer

    Submitted Jun 16 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up