Application
Other - Do not use
Review text: This peptide was used for dot blots to quality control anti-CTD antibodies. It was detected by ab5131 but not with anti-S2-CTD antibody.
Sample: Human Recombinant protein
Primary antibody (in addition to 'RNA polymerase II CTD repeat YSPTSPS peptide - phospho S5')
Dilution: 1/3000
Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/20000
Additional data
Notes: membranes were dotted with 0.1ug of peptide and blocked with 2% bsa at r/t for 1hr prior to primary antibody incubation
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted May 25 2012