Application
Other - Do not use
Review text: This peptide was used in an in vitro pulldown assay. It was biotinylated and linked to streptavidin coated magnetic beads. This complex was then mixed with our proteins of interest under a variety of conditions. Proteins which bound to the CTD-S5p peptide were eluted and assessed by western blot.
This peptide was used along side ab12793, ab12795, and an unrelated non-abcam peptide and binding to each CTD was compared.
Sample: Human Recombinant protein
Primary antibody (in addition to 'RNA polymerase II CTD repeat YSPTSPS peptide - phospho S5')
Primary antibody: None used
Secondary antibody
Secondary antibody: None used
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Aug 07 2012