Human RTK Phosphorylation Antibody Array -Membrane (71 Targets) (ab193662)

Overview

  • Product name

    Human RTK Phosphorylation Antibody Array -Membrane (71 Targets)
  • Sample type

    Cell Lysate, Tissue Lysate
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab193662 can be used for the simultaneous detection of 71 phosphorylated Human receptor tyrosine kinases (RTKs) in cell and tissue lysates. 


    Targets: ALB1, ACK1, ALK, Axl, Blk, BMX, Btk, Csk, Dtk, EGFR, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ErbB2, ErbB3, ErbB4, FAK, FER, FGFR1, FGFR2, FGFR2 (α isoform), Fgr, FRK, Fyn, Hck, HGFR, IGF-I R, Insulin R (CD220), Itk, JAK1, JAK2, JAK3, LCK, LTK, Lyn, MATK, M-CSFR, MUSK, NGFR (TNFRSF16), PDGFR-α, PDGFR-β, PYK2, RET, ROR1, ROR2, ROS, RYK, SCF R (CD117/c-kit), SRMS, SYK, Tec, Tie-1, Tie-2, TNK1, TRKB, TXK, Tyk2, TYRO10 (DDR2/TKT), VEGFR2, VEGFR3, ZAP70. 


    Learn more about membrane antibody arrays


     

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 2 Membranes 1 x 8 Membranes
    1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl
    1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml
    20X Wash Buffer I 1 x 30ml 1 x 30ml 2 x 30ml
    20X Wash Buffer II 1 x 30ml 1 x 30ml 2 x 30ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Biotinylated Anti-Phosphotyrosine Antibody (C1) 2 vials 1 vial 4 vials
    Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml
    Human RTK Antibody Array Membranes (C1) 1 x 4 units 1 x 2 units 1 x 8 units
    Phosphatase Inhibitor Cocktail Set II 1 x 60µl 1 x 60µl 2 x 60µl
    Protease Inhibitor Cocktail 1 x 60µl 1 x 60µl 2 x 60µl

Applications

Our Abpromise guarantee covers the use of ab193662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical images obtained with Abcam Human RTK Phosphorylation Antibody Array -Membrane (71 Targets) ab193662. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Protocols

References

This product has been referenced in:

  • Khaodee W  et al. Knockout of glucosidase II beta subunit inhibits growth and metastatic potential of lung cancer cells by inhibiting receptor tyrosine kinase activities. Sci Rep 9:10394 (2019). Read more (PubMed: 31316108) »
See 1 Publication for this product

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