Product nameHuman S100A1 Matched Antibody Pair Kit
See all S100 kits
Assay typeELISA set
Range62.5 pg/ml - 4000 pg/ml
Species reactivityReacts with: Human
Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human S100A1.
Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.
- Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.
- Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.
- Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.
Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab219635) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 2 x 96 tests Human S100A1 Capture Antibody 1 x 100µg 1 x 20µg Human S100A1 Detector Antibody 1 x 25µg 1 x 5µg Human S100A1 Lyophilized Protein 1 vial 1 vial
FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
- Information by UniProt
- Protein S100-A1
SimpleStep ELISA kits
Our Abpromise guarantee covers the use of ab221428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab221428 has not yet been referenced specifically in any publications.