Overview

  • Product name

    Human S100A12 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 0.49ng/ml 0.002 5.1%
    2 16 1.66ng/ml 0.097 5.9%
    3 16 8.37ng/ml 0.451 5.4%
    Inter-assay
    Sample n Mean SD CV%
    1 24 0.41ng/ml 0.022 5.4%
    2 24 1.89µg/ml 0.113 6%
    3 24 7.14ng/ml 0.414 5.8%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    31.2 pg/ml - 20000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human S100A12 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213822) is designed for the quantitative measurement of Human S100A12 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for S100A12 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human S100A12 amount of sample captured in plate.

  • Notes

    S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. It is also known as calgranulin C. By direct R-banding FISH, the S100A12 gene is mapped to chromosome 1q21.2-q22. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. And this protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human S100A12 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated S100A12 Antibody 1 x 130µl
    Lyophilized recombinant human S100A12 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Binds calcium, zinc and copper. Presence of zinc increases the affinity for calcium. Plays an important role in the inflammatory response. Interaction with AGER on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators.
  • Tissue specificity

    Monocytes and lymphocytes.
  • Sequence similarities

    Belongs to the S-101 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • Alternative names

    • CAAF1
    • CAGC
    • Calcitermin
    • Calcium-binding protein in amniotic fluid 1
    • Calgranulin C
    • Calgranulin-C
    • Calgranulin-related protein
    • CGRP
    • EN RAGE
    • EN-RAGE
    • ENRAGE
    • Extracellular newly identified RAGE-binding protein
    • migration inhibitory factor-related protein 6
    • MRP6
    • Neutrophil S100 protein
    • p6
    • Protein S100 A12
    • S100 calcium binding protein A12
    • S100 calcium-binding protein A12
    • S100 calcium-binding protein A12 (calgranulin C)
    • S100A12
    • S10AC_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human S100A12 ELISA Kit (ab213822) Standard Curve.

Protocols

References

ab213822 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213822.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up