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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Human S100A9 Antibody Pair - BSA and Azide free (ab241896)

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Sandwich ELISA - Human S100A9 Antibody Pair - BSA and Azide free (ab241896)

    Key features and details

    • Unconjugated capture and detector antibodies
    • Adaptable to any antibody pair-based assay format
    • Antibody concentration ~ 1 mg/ml
    • BSA and azide free buffer - ready for conjugation
    • Reacts with: Human

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    Overview

    • Product name

      Human S100A9 Antibody Pair - BSA and Azide free
      See all S100A9 kits
    • Assay type

      ELISA set
    • Range

      62.5 pg/ml - 4000 pg/ml
    • Species reactivity

      Reacts with: Human
    • Product overview

      The Antibody Pair can be used to quantify Human S100A9. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


      For additional information on the performance of the antibody pair, see the equivalent Matched Antibody Pair Kit (ab222271), which uses the same antibodies. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


      Download SDS here.

    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Carrier free

      Yes
    • Components 10 x 96 tests
      Human S100A9 Capture Antibody (unconjugated) 1 x 100µg
      Human S100A9 Detector Antibody (unconjugated) 1 x 100µg
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Calcium Binding Proteins
      • Cancer
      • Tumor biomarkers
      • Other
    • Function

      Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
    • Tissue specificity

      Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
    • Cellular localization

      Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
    • Target information above from: UniProt accession P06702 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Leukocyte L1 complex heavy chain
      • 60B8AG
      • CAGB
      • Calgranulin B
      • Calgranulin-B
      • Calprotectin L1H subunit
      • CFAG
      • CGLB
      • Cystic fibrosis antigen B
      • L1AG
      • Leukocyte L1 complex heavy chain
      • LIAG
      • MAC387
      • MIF
      • Migration inhibitory factor related protein 14
      • Migration inhibitory factor-related protein 14
      • MRP 14
      • MRP-14
      • MRP14
      • Myeloid-related protein 14
      • NIF
      • OTTHUMP00000015331
      • p14
      • Protein S100-A9
      • S100 A9
      • S100 calcium binding protein A9
      • S100 calcium binding protein A9 calgranulin B
      • S100 calcium-binding protein A9
      • S100A9
      • S10A9_HUMAN
      see all
    • Database links

      • Entrez Gene: 6280 Human
      • Omim: 123886 Human
      • SwissProt: P06702 Human
      • Unigene: 112405 Human

      Associated products

      • Alternative Versions

        • Human S100A9 Matched Antibody Pair Kit (ab222271)

      Applications

      Our Abpromise guarantee covers the use of ab241896 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Sandwich ELISA Use at an assay dependent concentration.

      Images

      • Sandwich ELISA - Human S100A9 Antibody Pair - BSA and Azide free (ab241896)
        Sandwich ELISA - Human S100A9 Antibody Pair - BSA and Azide free (ab241896)
        To learn more about the advantages of recombinant antibodies see here.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab241896? Please let us know so that we can cite the reference in this datasheet.

      ab241896 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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