Overview

  • Product name

    Human SCF ELISA Kit
    See all SCF kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    2.74 pg/ml - 2000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.3 85% - 106%
    Serum 94.29 80% - 103%
    Plasma 95.48 82% - 104%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat, Human
  • Product overview

    Abcam’s SCF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SCF in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human SCF coated on a 96-well plate. Standards and samples are pipetted into the wells and SCF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SCF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SCF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human Stem Cell Factor ELISA Kit (ab176109) from our SimpleStep ELISA® range


     

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human SCF (lyophilized) 2 vials
    Recombinant Human SCF Standard (lyophilized) 2 vials
    SCF Microplate (12 x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • Involvement in disease

    Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • Sequence similarities

    Belongs to the SCF family.
  • Developmental stage

    Acts in the early stages of hematopoiesis.
  • Post-translational
    modifications

    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • Cellular localization

    Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt
  • Alternative names

    • C kit ligand
    • C-kit ligand
    • Ckit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • KIT ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • MGF stem cell factor
    • SCF
    • SCF_HUMAN
    • SF
    • SHEP7
    • sKITLG
    • Soluble KIT ligand
    • Steel factor
    • steel, mouse, homolog of
    • Stem cell factor
    • Stem cell factor precursor
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human SCF measured in undiluted cell culture supernatants (duplicates +/- SD).

  • Human SCF measured in undiluted human biological fluids (duplicates +/- SD). Mouse and rat sera and plasma showed undetectable levels.

  • Standard curves in different buffers with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100636

  • Representative standard curve using ab100636

Protocols

References

This product has been referenced in:

  • Kim H  et al. Adipose-Derived Stem Cell Coculturing Stimulates Integrin-Mediated Extracellular Matrix Adhesion of Melanocytes by Upregulating Growth Factors. Biomol Ther (Seoul) 27:185-192 (2019). Read more (PubMed: 30530924) »
  • Feng J  et al. Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder. Mol Med Rep 16:1516-1522 (2017). Rat . Read more (PubMed: 28627603) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Sample: Cell-free supernatants from NIH 3T3 cell culture centrifuged at 15,000 rpm for 10 min at 4ºC. Samples were stored at -70ºC until the ELISAs were performed.
Amount of Sample: 50 ul
Type of Kit Used: ELISA
Manufacturer instructions were followed.
Additional data
We diluted 50 ul of supernatants in 50 ul of 1XAssay Diluent B
Washings were done on a Microplate Washer machine with 300 ul of wash buffer.
The standards data generated an excellent curve.
PubMed ID: 26522471

Abcam user community

Verified customer

Submitted Nov 21 2015

Question
Answer

Thank you very much for your phone call this morning.

As discussed I will be followingthis case further. Could you please provide the following details;

- Types of sample used. If supernatant, what type of cells were incubated
- Dilutions used for test samples
- the step altered in protocol, if any
- For how long primary and secondary antibodies were incubated?
- Were the assay diluents at room temperature when used?
- the purchase order number
- raw data

Please forward these details to me asap. I will be happy to look into the details and will try to solve the problem as soon as I can. Please also do not hesitate to contact me if you have any further question.

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