Overview

  • Product name
    Human SCF ELISA Kit
    See all SCF kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pooled Serum 24 6%
    Inter-assay
    Sample n Mean SD CV%
    Pooled Serum 6 2%
  • Sample type
    Serum, Plasma, Cell culture media, Heparin Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.51 pg/ml
  • Range
    15.63 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 102 92% - 111%
    Cell culture media 106 100% - 113%
    Heparin Plasma 105 91% - 115%
    Citrate Plasma 89 79% - 103%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Product overview

    Stem Cell Factor (SCF) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of SCF protein in Human plasma, serum and cell culture supernatant samples.


    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    SCF is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF is expressed in fibroblasts, liver cells, Sertoli cells, endothelial cells, neurons, macrophages, oocytes, Schwann cells and numerous carcinoma cell lines. SCF can be found as a cell membrane protein or as a soluble protein after secretion due to proteolytic processing in the extracellular domain. Both forms have growth factor activities; however the membrane form is also important for cell-cell adhesion and interaction.

    Soluble SCF is a heavily N- and O-glycosylated protein that exists as a non-covalently linked homodimer or as a heterotetramer with its receptor. Binding to the receptor, results in KIT activation by autophosphorylation. This leads to multiple signaling cascades (via AKT, GRB2, RAS, MAPK amongst others) that are important in the regulation of cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, melanogenesis and neurite-inductive activity of dorsal root ganglia neurons.

    Genetic defects in SCF are the cause of familial progressive hyperpigmentation (melanosis universalis hereditaria). SCF has also been involved in other conditions such as mastocytosis, mast cell leukemia, myeloid leukemia, aplastic anemia and gastrointestinal stromal tumor.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X SCF Capture Antibody 1 x 600µl
    10X SCF Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Plate Seals 1 unit
    Sample Diluent 50BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SCF Human Lyophilized Recombinant Protein 2 vials
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas
  • Function
    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • Involvement in disease
    Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • Sequence similarities
    Belongs to the SCF family.
  • Developmental stage
    Acts in the early stages of hematopoiesis.
  • Post-translational
    modifications
    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • Cellular localization
    Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt
  • Alternative names
    • C kit ligand
    • C-kit ligand
    • Ckit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • KIT ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • MGF stem cell factor
    • SCF
    • SCF_HUMAN
    • SF
    • SHEP7
    • sKITLG
    • Soluble KIT ligand
    • Steel factor
    • steel, mouse, homolog of
    • Stem cell factor
    • Stem cell factor precursor
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab176109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The levels of SCF in serum samples were tested from ten individual healthy donors. Levels were interpolated from the standard curve in Sample diluent 50BP and corrected for sample dilution. The levels of SCF are shown for the percentage of individuals within each 100 pg/mL bin center of the distribution. The mean level of SCF was 330 pg/mL with a range of 185 to 498 pg/mL and a standard deviation of 82 pg/mL.

Protocols

References

ab176109 has not yet been referenced specifically in any publications.

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