Overview

  • Product name

    Human SCGB1A1 ELISA Kit (uteroglobin)
    See all Uteroglobin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.2ng/ml 0.071 5.9%
    2 16 2.3ng/ml 0.085 3.7%
    3 16 5.2ng/ml 0.276 5.3%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1.6ng/ml 0.101 6.3%
    2 24 2.8ng/ml 0.154 5.5%
    3 24 6.1ng/ml 0.464 7.6%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human SCGB1A1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (uteroglobin) (ab213823) is designed for the quantitative measurement of Human SCGB1A1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for SCGB1A1/uteroglobin has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: E22-N91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCGB1A1/uteroglobin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SCGB1A1/uteroglobin amount of sample captured in plate.

  • Notes

    SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with anti-inflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its anti-inflammatory activities, UG manifests anti-chemotactic, anti-allergic, anti-tumorigenic, and embryonic growth stimulatory activities.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human SCGB1A1 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human SCGB1A1antibody 1 x 130µl
    Lyophilized recombinant Human SCGB1A1 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
  • Tissue specificity

    Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
  • Sequence similarities

    Belongs to the secretoglobin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Blastokinin
    • CC10
    • CC16
    • CCPBP
    • CCSP
    • Clara cell phospholipid binding protein
    • Clara cell phospholipid-binding protein
    • Clara cell specific 10 kD protein
    • Clara cells 10 kDa secretory protein
    • OTTHUMP00000236107
    • SCGB1A1
    • Secretoglobin family 1A member 1
    • Secretoglobin, family 1A, member 1 (uteroglobin)
    • UG
    • UGB
    • UP-1
    • UP1
    • Urinary protein 1
    • Urine protein 1
    • UTER_HUMAN
    • Uteroglobin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human SCGB1A1 ELISA Kit (uteroglobin) (ab213823) Standard Curve.

Protocols

References

ab213823 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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