Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Schistosoma mansoni IgG ELISA Kit
Intra-assay Sample n Mean SD CV% Pos. Serum 24 5.1% Inter-assay Sample n Mean SD CV% Pos. Serum 4 5.7%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Schistosoma mansoni IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Schistosoma mansoni in Human serum and plasma.
A 96-well plate has been precoated with Schistosoma mansoni antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidise (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Schistosoma mansoni-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Schistosoma mansoni IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Protein A HRP Conjugate Blue, Black cap 1 x 20ml Schistosoma mansoni (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Schistosoma mansoni IgG Cut-off Control Yellow, Green cap 1 x 3ml Schistosoma mansoni IgG Negative Control Yellow, Blue cap 1 x 2ml Schistosoma mansoni IgG Positive Control Yellow, Red cap 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
ab108769 has not yet been referenced specifically in any publications.