• Product name
    Human SDF1 alpha ELISA Kit
    See all SDF1 alpha kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall < 10%
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 80 pg/ml
  • Range
    93.8 pg/ml - 6000 pg/ml
  • Recovery

    > 80 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.63 86% - 104%
    Plasma 89.34 85% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human SDF1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100637) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SDF1 alpha in plasma and cell culture supernatants. (Human SDF1 alpha concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human SDF1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SDF1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect the endogenous Human SDF-1 alpha in normal serum with ab100637, only in serum spiked with Human SDF-1 alpha.

  • Notes

    Optimization may be required with urine samples.


  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human SDF1 alpha (lyophilized) 2 vials
    Recombinant Human SDF1 alpha Standard (lyophilized) 2 vials
    SDF1 alpha Microplate (12 x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.
  • Alternative names
    • C-X-C motif chemokine 12
    • Chemokine (C X C motif) ligand 12
    • CXCL12
    • hIRH
    • Intercrine reduced in hepatomas
    • IRH
    • PBSF
    • Pre B cell growth stimulating factor
    • SCYB12
    • SDF 1
    • SDF 1 beta
    • SDF 1b
    • SDF1
    • SDF1 alpha (3-67)
    • SDF1 beta (3-72)
    • SDF1A
    • SDF1B
    • Stromal cell derived factor 1
    • TLSF a
    • TLSF b
    • TLSFa
    • TLSFb
    • TPAR1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100637

  • Representative standard curve using ab100637



This product has been referenced in:
  • Cotoia A  et al. Circulating stem cells, HIF-1, and SDF-1 in septic abdominal surgical patients: randomized controlled study protocol. Trials 19:179 (2018). Read more (PubMed: 29530072) »
  • Miles B  et al. Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen. Infect Immun 82:101-11 (2014). Sandwich ELISA ; Human . Read more (PubMed: 24126519) »

See all 2 Publications for this product

Customer reviews and Q&As

The ELISA kit for SDF-1 alpha (aa 3-67) will detect the full length pro-SDF-1 (aa 1-93) and SDF-1 beta isoform (aa 3-72 ), albeit with a reduced affinity (approximately 25%). This has been tested empirically.

SDF1-CXCL12 human macrophage secretion

Excellent Excellent 5/5 (Ease of Use)
SDF1-CXCL12 secretion by human macrophages isolated by negative selection in culture for 3 or 7 days.
Kit easy to use, the assay takes about 5.30hr,
Secretion of CXCL12 by all different macrophage subtypes: M0(MCSF), M1(MSCF+IFNg), M2a (MSCF+IL4), M2c-DEX (MCSF+Dexamethasone), M2c-IL10(MCSF+IL10), M2c-TGFb(MCSF+TGFb)

Abcam user community

Verified customer

Submitted Apr 01 2014

If the background is already low though, there are a few options to increase the overall OD responses from your samples. The optional overnight standard/sample incubation is the easiest way but since you have already tried this some other methods are b...

Read More

I am happy to confirm that any of the 3 anti-coagulants should be fine to be used for testing plasma samples with the SDF1 alpha Human ELISA Kit (ab100637).

Merci de votre émail.

Nous allons donc ajouter un kit entier à votre prochaine commande. Est-ce que vous pouvez s'il vous plaît nous renvoyer le tube de HRP-Streptavidin?

Merci beaucoup.

Merci pour votre émail.

Je peux vous confirmer que le produit doit arriver demain chez vous. Le numéro de commande est le xx.

Je suis désolée du délais et des inconvenances. Veuillez ne pas h&eacut...

Read More

Merci de votre confirmation.

Nous sommes en train de procéder au remplacement et il va être ajouté à une de vos prochaines commandes. Nous allons vous confirmer laquelle dès que nous pouvons.

Merci ...

Read More

Merci de nous avoir contacté. Nous sommes en train d'organiser un remplacement. Est-ce que vous pouvez nous confirmer que le tube était bien centrifugé aussi pour vérifier que le liquid n'étais pas dans le capuchon? <...

Read More

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one kit of ab100637.
To check the status of the order please contact our Cus...

Read More

Congratulations on your new arrival!
I will send out that replacement kit of ab100637. As you are out of the lab, to whose attention should I send shipments to?

1-10 of 21 Abreviews or Q&A


Sign up