Overview

  • Product name

    Human SDMA ELISA kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample 1 12 0.27µM 7.5%
    Sample 2 12 0.67µM 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 6 0.22µM 6%
    Sample 2 6 0.63µM 7%
  • Sample type

    Serum, EDTA Plasma
  • Assay type

    Competitive
  • Sensitivity

    0.05 µM
  • Range

    0.001 µM - 1.1 µM
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Spike 101.5 101% - 102%

  • Assay time

    21h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human SDMA ELISA kit (ab213973) is intended for the quantitative determination of symmetric dimethylarginine (SDMA) in human EDTA-plasma and serum.


    This assay is based on the method of competitive enzyme linked immunoassays. The sample preparation includes the addition of a derivatization reagent for SDMA derivatization. Afterwards, the treated samples and the polyclonal SDMA antiserum are incubated in wells of a microtiter plate coated with SDMA derivative (tracer). During the incubation period, the target SDMA in the sample competes with the tracer immobilized on the wall of the microtiter wells for the binding of the polyclonal antibodies. The SDMA in the sample displaces the antibodies out of the binding to the tracer. Therefore the concentration of the tracer-bound antibody is inverse proportional to the SDMA concentration in the sample. During the second incubation step, a peroxidase conjugated antibody is added to each microtiter well to detect the anti-SDMA antibodies. After washing away the unbound components tetramethylbenzidine (TMB) is added as a peroxidase substrate. Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow and the absorbance is measured in a photometer at 450 nm. The intensity of the yellow color is inverse proportional to the SDMA concentration in the sample; this means high SDMA concentration in the sample reduces the concentration of tracer-bound antibodies and lowers the photometric signal.


    A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. SDMA present in the samples is determined directly from this curve.

  • Notes

    The dosage of most drugs must be adapted in renal insufficiency, making accurate assessment of renal function a prerequisite in clinical medicine Furthermore, even a modest decline in renal function has been recognized as a cardiovascular risk.

    In clinical practice serum creatinine is typically used to asses renal function, but this serum creatinine does not increase at modest decline in renal function. Consequently, there is an ongoing search for suitable endogenous markers of renal function.

    SDMA is a methylated derivative of L-Arginine which is strictly eliminated by renal extraction, thus SDMA plasma level is strongly correlated to renal function. In 18 studies with more than 2136 patients systemic SDMA concentrations correlated highly with inuline clearance, as well as with various clearance estimates combined and serum creatinine. With respect to this SDMA exhibits properties of a reliable marker of renal dysfunction.

    Moreover, there are hints that increased SDMA correlates with total sequential organ failure indicating both renal and hepatic failure and an increased cardiovascular risk.

    Indication

    • Renal failure
    • Cardiovascular risk in renal dysfunction
    • Hypertension in renal dysfunction
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    Conjugate 1 x 12ml
    Control 1 1 x 500µl
    Control 2 1 x 500µl
    Derivatization Reagent 1 x 6ml
    Reaction Buffer 1 x 15ml
    SDMA Antibody 1 x 6ml
    SDMA Coated Microplate (12x 8 well strips) 1 unit
    Standard 1 (0.0 µM) 1 x 500µl
    Standard 2 (0.1 µM) 1 x 500µl
    Standard 3 (0.3 µM) 1 x 500µl
    Standard 4 (0.6 µM) 1 x 500µl
    Standard 5 (1.5 µM) 1 x 500µl
    Standard 6 (4.0 µM) 1 x 500µl
    Stop Solution 1 x 15ml
    TMB Substrate 1 x 15ml
    Wash Buffer Concentrate (10X) 2 x 100ml
  • Alternative names

    • symmetric dimethylarginine

Applications

Our Abpromise guarantee covers the use of ab213973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab213973 has not yet been referenced specifically in any publications.

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