Overview

  • Product name
    Anti-Human Serum Albumin antibody [15C7]
    See all Human Serum Albumin primary antibodies
  • Description
    Mouse monoclonal [15C7] to Human Serum Albumin
  • Host species
    Mouse
  • Specificity
    No cross-reactivity with other human proteins tested.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Chicken, Cow
  • Immunogen

    Human serum albumin.

  • General notes

    Concentration varies from lot to lot and can be provided on request.

    This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.

Properties

Applications

Our Abpromise guarantee covers the use of ab10241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
ICC/IF Use a concentration of 2.5 µg/ml. PubMed: 19497803
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Human Serum Albumin (ab2406).

Target

  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • Analbuminemia antibody
    • Bisalbuminemia antibody
    • Cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • Dysalbuminemic hyperthyroxinemia antibody
    • Growth inhibiting protein 20 antibody
    • HSA antibody
    • Hyperthyroxinemia dysalbuminemic antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

Images

  • ab10241 staining Human Serum Albumin in human embryonic stem cell derived hepatocytes by immunocytochemistry/ immunofluorescence. Cells were methanol fixed and blocked in 10% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C. A FITC conjugated goat polyclonal to mouse IgG antibody, diluted 1/400, was used as the secondary (green). DAPI used to stain the nuclei (blue).

    See Abreview

  • Standard Curve for Human Serum Albumin (Analyte: Human Serum Albumin protein (ab67670)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) at 1µg/ml and Detector Antibody Rabbit polyclonal to Human Serum Albumin (ab2406) at 0.5µg/ml.

  • Detection of human serum albumin by ab10241 in western blotting. Lane 1 - human serum albumin, 3 µg. Lane 2 - normal human serum, ~3 µg of human serum albumin. Detection of human serum albumin by ab10241 in western blotting. Lane 1 - human serum albumin, 3 µg. Lane 2 - normal human serum, ~3 µg of human serum albumin.

References

This product has been referenced in:
  • Pewkliang Y  et al. A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax. Malar J 17:50 (2018). Read more (PubMed: 29370800) »
  • Song Y  et al. Patient-derived multicellular tumor spheroids towards optimized treatment for patients with hepatocellular carcinoma. J Exp Clin Cancer Res 37:109 (2018). Read more (PubMed: 29801504) »
See all 18 Publications for this product

Customer reviews and Q&As

1-10 of 23 Abreviews or Q&A

Answer



According to the testing data and Abreviews from customers on the datasheet, this antibody has been used successfully with various blocking agents including BSA. For example, there is an Abeview where BSA has been used as a blocking agent in WB:

https://www.abcam.com/Proteasome-subunit-beta-type-2-antibody-MCP165-ab22650/reviews/7252

However, please note protocols can sometimes require individual optimization.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hES derived hepatocytes)
Specification
hES derived hepatocytes
Fixative
Methanol
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 22 2012

Question
Answer

Thank you for contacting us.

I have heard back from the lab:
For ab2406, for use with human tissues, BSA may work but for other species it may crossreact.
For ab10241, it is not crossreacting with BSA.

Thus using BSA would be fine, but I might suggest trying this in a test experiment before doing the antibody array.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Vielen Dank für Ihren Anruf.
Tatsächlich hat eine meiner Kolleginnen mit der Turbidimetrie gearbeitet, und nach einiger Diskussion sind wir der Meinung, dass für Ihren Kunden Antikörper, die in ELISA und/oder IP funktionieren, geeignet sein sollte.
Ich habe Ihnen daher folgende Produkte heraus gesucht:
ab10241 Anti-Human Serum Albumin antibody [15C7]
ab104453 Anti-Lipoprotein a antibody
ab7616 Anti-Apolipoprotein B antibody
Allerdings habe ich nichts für das CRP gefunden.
Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.
Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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Answer

Thank you for your reply.

As requested, I have attached a copy of the CoC for ab10241 Human Serum Albumin antibody (15C7) including the lot number.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your enquiry.

Some of our products are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within 6 months of purchase. Some of our collaborators are not certified ISO9001, and so I am sorrywe are regrettably not able to provide a certificate of analysis for these particular products.

As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product.

The Customer Services and Scientific Support Teams would be very pleased to send a CoC on demand. Could you please let me know if you would like to receive a CoC?

Please do not hesitate to contact me again if you have further questions.

Read More

Question
Answer

Thank you very much for your interest in ab10241.

To our knowledge, ab10241 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab10241 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 freePRIMARY ANTIBODYOR $330.00 OFF OF NEXT ORDER.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab10241 in IHC-P. I will then send a discount code. This code must be issued before purchasingab10241 so please wait for my reply before ordering.

2. Purchase ab10241 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-P.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibodyordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10241 turns out to be unsuitable forIHC-Pyou will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your enquiry.

I am sorry the immunogen used to create ab10241 Human Serum Albumin antibody [15C7] is not available. In theory,it could be used with ab67670 Human Serum Albumin protein, however you will need to consider that this is a recombinant protein, not endogenous, and has not been tested together with the antibody in the laboratory.

I am sorry this is not better news for you on this occasion. If you have any further questions, please do not hesitate to contact us.

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Answer

Vielen Dank für Ihren Anruf.

Ich habe mich mit dem Labor in Verbindung gesetzt, welches diesen Antikörper herstellt. Leider wurde die leichte Kette nicht bestimmt.

"Unfortunately we have not determined if this MAb is a kappa or lambda chain. I am sorry for the inconvenience.
"

Ich hoffe, dies hilft Ihnen trotzdem weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

Please find attached certificate of compliance. We do not provide certificate of analysis for antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

1-10 of 23 Abreviews or Q&A

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