Anti-Human Serum Albumin antibody [15C7] (ab10241)


  • Product name
    Anti-Human Serum Albumin antibody [15C7]
    See all Human Serum Albumin primary antibodies
  • Description
    Mouse monoclonal [15C7] to Human Serum Albumin
  • Host species
  • Specificity
    No cross-reactivity with other human proteins tested.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Chicken, Cow
  • Immunogen

    Human serum albumin.

  • General notes

    Concentration varies from lot to lot and can be provided on request.

    This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.



Our Abpromise guarantee covers the use of ab10241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
ICC/IF Use a concentration of 2.5 µg/ml. PubMed: 19497803
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Human Serum Albumin (ab2406).

For sandwich ELISA, use this antibody as capture at 1 µg/ml with rabbit polyclonal to Human Serum Albumin (ab2406) as detection.

ab10241 can also be used as capture antibody with mouse monoclonal to Human Serum Albumin (ab37989) or HRP conjugated mouse monoclonal to Human Serum Albumin (ab24458) as a detection antibody.


  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
  • Involvement in disease
    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • Analbuminemia antibody
    • Bisalbuminemia antibody
    • Cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • Dysalbuminemic hyperthyroxinemia antibody
    • Growth inhibiting protein 20 antibody
    • HSA antibody
    • Hyperthyroxinemia dysalbuminemic antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all


  • ab10241 staining Human Serum Albumin in human embryonic stem cell derived hepatocytes by immunocytochemistry/ immunofluorescence. Cells were methanol fixed and blocked in 10% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C. A FITC conjugated goat polyclonal to mouse IgG antibody, diluted 1/400, was used as the secondary (green). DAPI used to stain the nuclei (blue).

    See Abreview

  • Standard Curve for Human Serum Albumin (Analyte: Human Serum Albumin protein (ab67670)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) at 1µg/ml and Detector Antibody Rabbit polyclonal to Human Serum Albumin (ab2406) at 0.5µg/ml.

  • Detection of human serum albumin by ab10241 in western blotting. Lane 1 - human serum albumin, 3 µg. Lane 2 - normal human serum, ~3 µg of human serum albumin. Detection of human serum albumin by ab10241 in western blotting. Lane 1 - human serum albumin, 3 µg. Lane 2 - normal human serum, ~3 µg of human serum albumin.


This product has been referenced in:
  • Pewkliang Y  et al. A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax. Malar J 17:50 (2018). Read more (PubMed: 29370800) »
  • Song Y  et al. Patient-derived multicellular tumor spheroids towards optimized treatment for patients with hepatocellular carcinoma. J Exp Clin Cancer Res 37:109 (2018). Read more (PubMed: 29801504) »

See all 16 Publications for this product

Customer reviews and Q&As

According to the testing data and Abreviews from customers on the datasheet, this antibody has been used successfully with various blocking agents including BSA. For example, there is an Abeview where BSA has been used as a blocking agent in ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (hES derived hepatocytes)
hES derived hepatocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 22 2012

Thank you for contacting us.

I have heard back from the lab:
For ab2406, for use with human tissues, BSA may work but for other species it may crossreact.
For ab10241, it is not crossreacting with BSA.

Thus using BSA would be ...

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Vielen Dank für Ihren Anruf.
Tatsächlich hat eine meiner Kolleginnen mit der Turbidimetrie gearbeitet, und nach einiger Diskussion sind wir der Meinung, dass für Ihren Kunden Antikörper, die in ELISA und/oder IP funktionieren, geeignet sein sollte...

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Thank you for your reply.

As requested, I have attached a copy of the CoC for ab10241 Human Serum Albumin antibody (15C7) including the lot number.

I hope this will be helpful. If you have any further questions, please do not hesi...

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Thank you for your enquiry.

Some of our products are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our A...

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Thank you very much for your interest in ab10241.

To our knowledge, ab10241 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab10241 now, test it in IHC-P and submit feedback to us in the form ...

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Thank you for your enquiry.

I am sorry the immunogen used to create ab10241 Human Serum Albumin antibody [15C7] is not available. In theory,it could be used with ab67670 Human Serum Albumin protein, however you will need to consider that thi...

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Vielen Dank für Ihren Anruf.

Ich habe mich mit dem Labor in Verbindung gesetzt, welches diesen Antikörper herstellt. Leider wurde die leichte Kette nicht bestimmt.

"Unfortunately we have not determined if this MAb is a kappa or ...

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Thank you for contacting us.

Please find attached certificate of compliance. We do not provide certificate of analysis for antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any mor...

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