Anti-Human Serum Albumin antibody [15C7] (ab10241)
![Dot Blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)](https://www.abcam.com/ps/products/10/ab10241/Images/ab10241-472611-HSA-AB--BSA-protein-00210241.jpg "Dot Blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)")
Key features and details
- Mouse monoclonal [15C7] to Human Serum Albumin
- Suitable for: WB, Sandwich ELISA, Dot blot
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-Human Serum Albumin antibody [15C7]
See all Human Serum Albumin primary antibodies -
Description
Mouse monoclonal [15C7] to Human Serum Albumin -
Host species
Mouse -
Specificity
No cross-reactivity with other human proteins tested. Does not react with bovine serum albumin, egg white albumin or human alpha fetoprotein.
-
Tested applications
Suitable for: WB, Sandwich ELISA, Dot blotmore details -
Species reactivity
Reacts with: Human
Does not react with: Chicken, Cow -
Immunogen
Full length native protein (purified) corresponding to Human Serum Albumin.
Database link: P02768 -
General notes
Concentration varies from lot to lot and can be provided on request.
This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
15C7 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
-
sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
|
| Sandwich ELISA |
Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Human Serum Albumin (ab2406).
|
|
| Dot blot |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa. |
|
Sandwich ELISA
Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Human Serum Albumin (ab2406). |
|
Dot blot
Use at an assay dependent concentration. |
Target
-
Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
-
Alternative names
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
Images
-
Dot Blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)
Dot Blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)
Lane 1: HSA 0.5 mg/ml
Lane 2: HSA 0.25 mg/ml
Lane 3: HSA 0.1 mg/ml
Lane 4: HSA 0.01 mg/ml
Lane 5: BSA 0.5 mg/ml
-
![Western blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)](https://www.abcam.com/ps/products/10/ab10241/Images/ab10241-260509-anti-human-serum-albumin-antibody-15c7-western-blot.jpg)
Western blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)This image is courtesy of an Abreview submitted by John AndersenAnti-Human Serum Albumin antibody [15C7] (ab10241) at 1/2000 dilution (in 5% milk for 1 hour) + Human blood serum at 2 µg
Secondary
anti-mouse IgG (H&L)
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 5 seconds
-
![Western blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)](https://www.abcam.com/ps/products/10/ab10241/Images/ab10241-17837-anti-human-serum-albumin-antibody-15c7-western-blot.jpg)
Western blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)Detection of Human Serum Albumin by ab10241 in western blotting.
Lane 1 - human serum albumin, 3 µg.
Lane 2 - normal human serum, ~3 µg of human serum albumin.
-
![Sandwich ELISA - Anti-Human Serum Albumin antibody [15C7] (ab10241)](https://www.abcam.com/ps/products/10/ab10241/Images/ab10241-17838-anti-human-serum-albumin-antibody-15c7-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Human Serum Albumin antibody [15C7] (ab10241)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (33)
ab10241 has been referenced in 33 publications.
- Taymour R et al. 3D bioprinting of hepatocytes: core-shell structured co-cultures with fibroblasts for enhanced functionality. Sci Rep 11:5130 (2021). PubMed: 33664366
- Dimov N et al. Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay. J Vis Exp N/A:N/A (2020). PubMed: 32150159
- Gregory JV et al. Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy. Nat Commun 11:5687 (2020). PubMed: 33173024
- Kongmanas K et al. Immortalized stem cell-derived hepatocyte-like cells: An alternative model for studying dengue pathogenesis and therapy. PLoS Negl Trop Dis 14:e0008835 (2020). PubMed: 33216752
- Pez F et al. In vitro transformation of primary human hepatocytes: Epigenetic changes and stemness properties. Exp Cell Res 384:111643 (2019). PubMed: 31557464