Overview

  • Product name
    Anti-Human Serum Albumin antibody
    See all Human Serum Albumin primary antibodies
  • Description
    Rabbit polyclonal to Human Serum Albumin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Sandwich ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human albumin.

  • Positive control
    • IHC-P: Human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab2406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [15C7] to Human Serum Albumin (ab10241). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) as Capture.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
This antibody may be diluted to a titer of 1/50-1/1000 in an ABC method. We suggest an incubation period of 30 minutes at room temperature.

Target

  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • Analbuminemia antibody
    • Bisalbuminemia antibody
    • Cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • Dysalbuminemic hyperthyroxinemia antibody
    • Growth inhibiting protein 20 antibody
    • HSA antibody
    • Hyperthyroxinemia dysalbuminemic antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Human Serum Albumin using ab2406 at 1/50 dilution in immunohistochemical analysis.

  • Ab2406 staining human normal liver. Staining is localised to the cytoplasm and secreted.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be re
  • Standard Curve for Human Serum Albumin (Analyte: Human Serum Albumin protein (ab67670)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) at 1µg/ml and Detector Antibody Rabbit polyclonal to Human Serum Albumin (ab2406) at 0.5µg/ml.

References

This product has been referenced in:
  • Zheng Z  et al. Hepatitis C virus mediated chronic inflammation and tumorigenesis in the humanised immune system and liver mouse model. PLoS One 12:e0184127 (2017). Read more (PubMed: 28886065) »
  • Lichtmannegger J  et al. Methanobactin reverses acute liver failure in a rat model of Wilson disease. J Clin Invest 126:2721-35 (2016). Read more (PubMed: 27322060) »
See all 12 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (skeletal muscle)
Gel Running Conditions
Reduced Denaturing (10% Gel)
Loading amount
30 µg
Specification
skeletal muscle
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 13 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skeletal muscle)
Permeabilization
No
Specification
skeletal muscle
Fixative
none

Abcam user community

Verified customer

Submitted May 28 2018

Answer

Thank you for your enquiry and your interest.

I can confirm that the immunogen used to raise this antibody was a full length purified human albumin. We do not have any cross-reactivity data with other species but a soon as we have further information, we will update our datasheet accordingly.

The average Ig concentration for this antibody is 3.3 mg/ml unless you have specific batch you are requesting.

If you need any further assistance in the future, please do not hesitate to contact me.

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Question
Answer

Thank you for contacting us.

I have heard back from the lab:
For ab2406, for use with human tissues, BSA may work but for other species it may crossreact.
For ab10241, it is not crossreacting with BSA.

Thus using BSA would be fine, but I might suggest trying this in a test experiment before doing the antibody array.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you very much for your interest in ab10241.

To our knowledge, ab10241 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab10241 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 freePRIMARY ANTIBODYOR $330.00 OFF OF NEXT ORDER.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab10241 in IHC-P. I will then send a discount code. This code must be issued before purchasingab10241 so please wait for my reply before ordering.

2. Purchase ab10241 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-P.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibodyordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10241 turns out to be unsuitable forIHC-Pyou will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your enquiry regarding ab2406.

The protein concentration for this antibody is 3.3 g/L. I regret to inform you that we do not have any information that explains how many mg of albumin can be bound per ug of antibody.

Since this product has never beencharacterziedin IP, I could offer you a testing discount if you wish to test it. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Ces trois anticorps que vous avez cité dans votre émail - ab31657, ab2406 et ab34856 - ne sont pas testés en Western blot. Néanmoins, si vous souhaitez tester un de ces trois anticorps, je vous conseillerais le ab2406 car il est au moins testé aussi dans l'immunohistochimie et non seulement dans l'ELISA. Cet anticorps (comme les deux autres) sont aussi en stock actuellement. Si vous souhaitez tester ab2406 en votre application ainsi que en Western blot en parallele, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab2406 pourra être utilisable lors d’une prochaine commande d’anticorps primaire. N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle si ceci peux vous intéresser.

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Question
Answer

DISCOUNT CODE: *** Expiration date: April 5th, 2012 I am very pleased to hear you would like to accept our offer and test ab2406 in WB. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for WB and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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