Overview

  • Product name

    Anti-Human Serum Albumin antibody
    See all Human Serum Albumin primary antibodies
  • Description

    Rabbit polyclonal to Human Serum Albumin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Goat, Horse, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (LSEKERQIKK QTALVELVKH KPKATKEQLK AVMDDFAAFV EKCCKADDKE) of Human Serum Albumin (NP_000468).

  • Positive control

    • Fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • Analbuminemia antibody
    • Bisalbuminemia antibody
    • Cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • Dysalbuminemic hyperthyroxinemia antibody
    • Growth inhibiting protein 20 antibody
    • HSA antibody
    • Hyperthyroxinemia dysalbuminemic antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

Images

  • Anti-Human Serum Albumin antibody (ab83465) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 69 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Human Serum Albumin with ab83465 at 5 µg/ml.

References

This product has been referenced in:

  • Newberry EP  et al. Hepatocyte and stellate cell deletion of liver fatty acid binding protein reveals distinct roles in fibrogenic injury. FASEB J N/A:fj201801976R (2018). Read more (PubMed: 30576225) »
  • Wang Y  et al. Association of calreticulin expression with disease activity and organ damage in systemic lupus erythematosus patients. Exp Ther Med 13:2577-2583 (2017). Read more (PubMed: 28565882) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Serum (Blood)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Blood
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Christopher Trummer

Verified customer

Submitted Oct 04 2018

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