• Product name
    Anti-Human Serum Albumin antibody
    See all Human Serum Albumin primary antibodies
  • Description
    Sheep polyclonal to Human Serum Albumin
  • Host species
  • Tested applications
    Suitable for: IHC-Fr, Double Immunodiffusion, Immunoelectrophoresis, RID, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig
  • Immunogen

    Human serum albumin purified from Cohn Fraction V.



Our Abpromise guarantee covers the use of ab8940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Double Immunodiffusion Use at an assay dependent concentration. 10µL antiserum vs 5µL serum (1/20 dilution).
Immunoelectrophoresis Use at an assay dependent concentration.

2µL antiserum/cm² gel vs 5µL 1/200 - 1/2000 dilution human serum IEP: 100µL antiserum vs 1µL serum The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily be excluded.

RID Use at an assay dependent concentration.
IHC-P 1/1000. See Abreview.
IHC-FoFr Use at an assay dependent concentration.



  • ab8940 at 1/1000 staining mouse embryonic tissue sections (E10.5) by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with 3ml maleate bufer, 1ml FCS, 1ML 10% blocking powder before incubation with the primary antibody for 16 hours at 37°C. A Cy3® conjugated donkey anti-sheep antibody was used as the secondary.

    The image shows hepatoblasts in the liver bud positive for albumin (red) and also some expressing E-cadherin (green).

    See Abreview

  • Immunohistochemical analysis of AAV2/8 injected rat liver tissue, staining Human Serum Albumin (red) with ab8940.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100, and blocked with 5% normal rabbit serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) overnight. An AlexaFluor®594-conjugated anti-sheep IgG (1/1000) was used as the secondary antibody.


This product has been referenced in:
  • Saito Y  et al. Induction of differentiation of intrahepatic cholangiocarcinoma cells to functional hepatocytes using an organoid culture system. Sci Rep 8:2821 (2018). Read more (PubMed: 29434290) »
  • Orbach SM  et al. High-throughput toxicity testing of chemicals and mixtures in organotypic multi-cellular cultures of primary human hepatic cells. Toxicol In Vitro 51:83-94 (2018). Read more (PubMed: 29751030) »

See all 25 Publications for this product

Customer reviews and Q&As

Thank you for your message.

I am sorry to hear you have continued to experience difficulties, butampleased you have found the source of the problem. Was the Cy5 anti sheep antibody from Abcam? Do you have the product code, and I will be plea...

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Thank you very much for your interest in ab10241.

To our knowledge, ab10241 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab10241 now, test it in IHC-P and submit feedback to us in the form ...

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Lamentablemente no puedo asegurarte que el anticuerpo vaya a funcionar en WB. Nosotros no lo hemos testado en el laboratorio, por lo que no tenemos evidencia de que vaya a reconocer la proteína en esta aplicación.

Siento no pode...

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Gracias por tu respuesta.

No tenemos un protocolo concreto para el uso de este anticuerpo mediante immunohistoquímica, sino protocolos generales que pueden servir de guía para los distintos ensayos.

Te adjunto el link ...

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Thank you for your enquiry.

I can confirm that this particular product, ab8940,is sourced externally. Using zone electrophoresis under standard conditions, the originator has shown that only sheep IgG is present, however if more sensitive te...

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Thank you for your message.

I can confirm you can quote the discount code previously provided to you when you place the order:

As requested, as a gesture of goodwill, I have set up a 50% discount which is valid for the purchase of...

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Thank you for your message.

I am sincerely sorry we did not manage to resolve this issue for you. The originator did not have any further information to provide on this occasion. As previously discussed, this antibody has not been tested in ...

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Gracias por tu respuesta.
Te confirmo que ab53435 solo se ha testado en secciones congeladas. No tenemos, pues, ninguna evidencia de que vaya a detectar el epítopo en secciones incluidas en parafina.
Así pues, puedes optar por testarlo en est...

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Gracias por tu respuesta.

Como bien indicas, ab53435 solo se ha testado en secciones congeladas, no tengo constancia de que funcione en parafina. De todas maneras, he contactado con mis compañeros del laboratorio para que me lo confirm...

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Muchas gracias por contactarnos de nuevo.

Lamentablemente, ya que tenemos en el catálogo más de 80.000 productos, nos resulta imposible guardar muestras de prueba de todos ellos para enviar como alícuotas de test.
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1-10 of 21 Abreviews or Q&A


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