Overview

  • Product name
    Anti-Human Serum Albumin antibody
    See all Human Serum Albumin primary antibodies
  • Description
    Sheep polyclonal to Human Serum Albumin
  • Host species
    Sheep
  • Tested applications
    Suitable for: IHC-Fr, Double Immunodiffusion, Immunoelectrophoresis, RID, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig
  • Immunogen

    Human serum albumin purified from Cohn Fraction V.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab8940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Double Immunodiffusion Use at an assay dependent concentration. 10µL antiserum vs 5µL serum (1/20 dilution).
Immunoelectrophoresis Use at an assay dependent concentration.

2µL antiserum/cm² gel vs 5µL 1/200 - 1/2000 dilution human serum IEP: 100µL antiserum vs 1µL serum The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily be excluded.

RID Use at an assay dependent concentration.
IHC-P 1/1000. See Abreview.
IHC-FoFr Use at an assay dependent concentration.

Target

Images

  • ab8940 at 1/1000 staining mouse embryonic tissue sections (E10.5) by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with 3ml maleate bufer, 1ml FCS, 1ML 10% blocking powder before incubation with the primary antibody for 16 hours at 37°C. A Cy3® conjugated donkey anti-sheep antibody was used as the secondary.

    The image shows hepatoblasts in the liver bud positive for albumin (red) and also some expressing E-cadherin (green).

    See Abreview

  • Immunohistochemical analysis of AAV2/8 injected rat liver tissue, staining Human Serum Albumin (red) with ab8940.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100, and blocked with 5% normal rabbit serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) overnight. An AlexaFluor®594-conjugated anti-sheep IgG (1/1000) was used as the secondary antibody.

References

This product has been referenced in:
  • Saito Y  et al. Induction of differentiation of intrahepatic cholangiocarcinoma cells to functional hepatocytes using an organoid culture system. Sci Rep 8:2821 (2018). Read more (PubMed: 29434290) »
  • Orbach SM  et al. High-throughput toxicity testing of chemicals and mixtures in organotypic multi-cellular cultures of primary human hepatic cells. Toxicol In Vitro 51:83-94 (2018). Read more (PubMed: 29751030) »
See all 30 Publications for this product

Customer reviews and Q&As

1-10 of 21 Abreviews or Q&A

Answer

Thank you for your message.

I am sorry to hear you have continued to experience difficulties, butampleased you have found the source of the problem. Was the Cy5 anti sheep antibody from Abcam? Do you have the product code, and I will be pleased to investigate this further.

In the case of the discount, I have changed thissoyou can have a50% discount on one vial ofeither a primary antibody or a secondary antibody. Although primary antibodies are usually more expensive.

I hope this will be helpful. Please do not hesitate to contact me if you have any further questions or concerns.

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Question
Answer

Thank you very much for your interest in ab10241.

To our knowledge, ab10241 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab10241 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 freePRIMARY ANTIBODYOR $330.00 OFF OF NEXT ORDER.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab10241 in IHC-P. I will then send a discount code. This code must be issued before purchasingab10241 so please wait for my reply before ordering.

2. Purchase ab10241 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-P.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibodyordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10241 turns out to be unsuitable forIHC-Pyou will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Lamentablemente no puedo asegurarte que el anticuerpo vaya a funcionar en WB. Nosotros no lo hemos testado en el laboratorio, por lo que no tenemos evidencia de que vaya a reconocer la proteína en esta aplicación.

Siento no poder ser de más ayuda, si necesitas cualquier otra información, no dudes en volver a contactarnos.

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Answer

Gracias por tu respuesta.

No tenemos un protocolo concreto para el uso de este anticuerpo mediante immunohistoquímica, sino protocolos generales que pueden servir de guía para los distintos ensayos.

Te adjunto el link del protocolo de immunohistoquímica para secciones incluidas en parafina:

https://www.abcam.com/index.html?pageconfig=resource&rid=11384

Así como el “recetario” de los distintos pasos a seguir:

https://www.abcam.com/index.html?pageconfig=resource&rid=13046

También te pueden resultar útiles los consejos para tratar los posibles problemas que pueden darse a lo largo del ensayo:

https://www.abcam.com/index.html?pageconfig=resource&rid=11467

Las únicas recomendaciones concretas para el uso de estos anticuerpos es utilizar como agente de bloqueo suero de conejo (especie de procedencia del secundario) y usar el primario a una dilución de 1/1000. Quizás te ayude echar un vistazo al Abreview publicado por otro usuario del anticuerpo ab8940 para comprobar en qué condiciones usó el anticuerpo.

Es importante también usar siempre controles positivos para ayudar a determinar el origen de los posibles problemas, así como llevar a cabo controles no primarios (incubando únicamente el secundario).

En cualquier caso, ambos anticuerpos están cubiertos por nuestra garantía Abpromise, y si siguieras teniendo problemas con ellos te ruego que nos contactes para ayudarte a resolverlos.

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Answer

Thank you for your enquiry.

I can confirm that this particular product, ab8940,is sourced externally. Using zone electrophoresis under standard conditions, the originator has shown that only sheep IgG is present, however if more sensitive techniques are used there may be additional proteins present. I am sorry they are regrettablynot able to provide an image on this occasion.

With regards to shipping to Poland, this information can be found on the online datasheet. I can recommend to select 'Poland' from the dropdown menu in the top right corner of the webpage. It should then provide estimated delivery times.

I have just checked this, and at the moment it states if you order it now, you would get it Thursday. So if you order today or tomorrow, you should get it Thursday or Friday this week.

I hope this will be helpful. if you have any further questions, please do not hesitate to contact us.

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Question

Hello,

Thanks for your reply.

We will pay for the new antibodies with the 50% discount.

How do we get this discount? Is there an identifier that need to be included in the order?

Also, are we able to obtain antibodies derived from the same batch as we ordered previously. This might be important because we are trying to reconstruct what we have done as closely as possible.

Regards



Robert Wilson







From: technical@abcam.com [technical@abcam.com]
Sent: 06 February 2012 10:49
To: Wilson, Robert
Subject: Reply from Abcam to your enquiry regarding ab8940, ab30973, ab96946 [CCE3485016]





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Dear Dr Wilson



We have an answer to your inquiry:



Thank you for your message.



Reviewing the details, I am sorry that we are not able to provide a replacementif the antibody has not been used in a tested application. In this case, the 50% discount is still applicable and I hope this will be helpful to you.



If you have any further questions or concerns, please do not hesitate to contact us.





Help us improve our service.
https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=3485016




Your original inquiry to Abcam:






Hello,







We have successfully used the antibodies with magnetic beads and a different primary antibody (beads coated with human serum albumin (HSA) and anti-HSA antibodies from Abcam as the primar antibody. Unfortunately our purchase of the Dylight anti-sheep antibodies was more than six months from present, although weoriginally contacted Abcamless than six month after the antibodies were purchased. I suppose than means we only get the 50% discount?







Robert Wilson














Best regards,
Kate

Kate Hayes
Scientific Support Supervisor
Abcam plc
www.abcam.com



Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

[CCE3485016]









Discover more at abcam.com

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Answer

Thank you for your message.

I can confirm you can quote the discount code previously provided to you when you place the order:

As requested, as a gesture of goodwill, I have set up a 50% discount which is valid for the purchase of one vial of primary antibody. Please provide this code to receive a 50% discount when you place your next order for a primary antibody from us.

Discount code: #####
Expiry date: ######

For lot numbers, again when you place your order please request the specific lot number within your order request. I am sorry I am not sure which specific antibodies you would be ordering, so I am not able to check the stock for you. However, when you place an order you would be contacted before the order is verified if any of the lot numbers you requestarenot available.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your message.

I am sincerely sorry we did not manage to resolve this issue for you. The originator did not have any further information to provide on this occasion. As previously discussed, this antibody has not been tested in this application before. Therefore, regrettably we cannot cover this antibody with our guarantee for this particular application on this occasion.

As requested, as a gesture of goodwill,I have set up a 50% discount which is valid for the purchase of one vial of primary antibody. Please provide this codeto receive a 50%discount when youplace your next order fora primary antibody fromus.

Discount code: #####
Expiry date: #######

If possible, I can recommend to consider trying the antibody in a tested application for which it will be covered by the 6 month guarantee. If it does not work in tested applications and species, please do not hesitate to let me know aswe can provide a full credit note or replacement.

I hope this will be helpful to you. If you have any further questions or concerns, please do not hesitate to contact me.

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Answer

Gracias por tu respuesta.
Te confirmo que ab53435 solo se ha testado en secciones congeladas. No tenemos, pues, ninguna evidencia de que vaya a detectar el epítopo en secciones incluidas en parafina.
Así pues, puedes optar por testarlo en esta aplicación, indicándomelo previamente para que yo te envíe el código canjeable por un anticuerpo gratuito, u optar por usar ab8940.
Si eliges la última opción te aconsejo que uses el método de recuperación antigénica que te suele funcionar. Yo particularmente aconsejo Tris EDTA en microondas, optimizando el tiempo de recuperación, es decir, probando a varios tiempos, a partir de un minuto, y no más de 20, para evitar dañar la morfología del tejido.
Como agente de bloqueo, la recomendación es usar suero de la misma especie en la que se generó el secundario, para evitar uniones inespecíficas de éste. Si utilizas el Rabbit anti-Sheep IgG H&L (FITC) secondary antibody (ab6743), lo ideal sería usar suero de conejo.
Espero haberte ayudado. Recuerda que si optas por probar ab53435 en IHC-P, el código debe emitirse en primer lugar, por lo que antes de hacer el pedido te pido que me lo indiques para enviarte dicho código.
Si tienes alguna otra consulta, no dudes en volver a contactarnos.

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Answer

Gracias por tu respuesta.

Como bien indicas, ab53435 solo se ha testado en secciones congeladas, no tengo constancia de que funcione en parafina. De todas maneras, he contactado con mis compañeros del laboratorio para que me lo confirmen, por si tuvieran algún dato o certeza acerca de su uso en esta aplicación.

Si no lo han testado antes en secciones de parafina, y si te interesa participar en nuestro sistema de evaluación de anticuerpos (Abreview), lo que puedes hacer, es testarlo en esta aplicación, y compartir los resultados en nuestra web, independientemente de que éstos sean positivos o negativos. Se trata de un modo de ampliar la información acerca de nuestros productos en su uso con aplicaciones o especies no testadas hasta el momento. A cambio de compartir los resultados se os ofrece un nuevo anticuerpo completamente gratuito.

Espero a saber la respuesta de mis compañeros del laboratorio, y si estáis interesados, os animo a leer acerca de los Abreviews, por si quisierais participar:

https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews

Si prefieres usar ab8940, que si se ha testado en secciones incluidas en parafina, y por tanto está garantizado (y en caso de no funcionar os enviaríamos un reemplazo o reembolso), simplemente aseguraros de usar un buen agente de bloqueo (se recomienda usar suero de la misma especia de procedencia del secundario, en este caso conejo), y un buen método probado de recuperación antigénica.

Lamento no poder ofrecer muestras gratuitas, pero espero que las opciones ofrecidas os puedan interesar. Espero tu respuesta, y en cuanto me contesten mis compañeros acerca del uso de ab53435 en parafina os vuelvo a contactar.

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Answer

Muchas gracias por contactarnos de nuevo.

Lamentablemente, ya que tenemos en el catálogo más de 80.000 productos, nos resulta imposible guardar muestras de prueba de todos ellos para enviar como alícuotas de test.

Sin embargo, lo que sí ofrecemos a cambio es una garantía en todos nuestros productos de 6 meses (Abpromise), que asegura que si un producto se ha usado siguiendo las especificaciones de la datasheet, y no funcionara, en ese caso ofreceremos un reemplazo gratuito, un reembolso o un vale para una compra futura.

Al no poder mandar muestras de prueba esta garantía permite a los usuarios confiar en que en caso de no funcionar el anticuerpo, al menos no habrá perdido el dinero.

Si tenéis cualquier otra consulta, por favor, no dudéis en volvernos a contactar.

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1-10 of 21 Abreviews or Q&A

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