Key features and details
- Mouse monoclonal [AL-01] to Human Serum Albumin (Biotin)
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Product nameAnti-Human Serum Albumin antibody [AL-01] (Biotin)
See all Human Serum Albumin primary antibodies
DescriptionMouse monoclonal [AL-01] to Human Serum Albumin (Biotin)
SpecificityReacts only with Human Serum Albumin. No cross-reactivity was observed with other serum proteins.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Does not react with: Mammals
Fraction of proteins containing Albumin after ammonnium sulphate precipitation and DEAE-chromatography of Human serum.
- WB: Human plasma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab81426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 69 kDa.|
FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid.
- Information by UniProt
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
All lanes : Anti-Human Serum Albumin antibody [AL-01] (Biotin) (ab81426) at 5 µg/ml
Lane 1 : Human plasma (non-reducing conditions)
Lane 2 : Human plasma (reducing conditions)
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate (reducing conditions)
All lanes : IRDye800-conjugated Streptavidin
Predicted band size: 69 kDaThe experiment was conducted under reducing and non-reducing conditions.Negative control: HeLa cell lysate.
ab81426 has been referenced in 1 publication.
- Edwards W et al. Targeting the ion channel Kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-life. J Biol Chem 289:22704-14 (2014). ELISA . PubMed: 24939846