Key features and details
- Rabbit polyclonal to Human Serum Albumin (Biotin)
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Human Serum Albumin antibody (Biotin)
See all Human Serum Albumin primary antibodies
DescriptionRabbit polyclonal to Human Serum Albumin (Biotin)
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human Human Serum Albumin.
- ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% Polyethylene glycol
Concentration information loading...
PurityProtein A purified
Purification notesPurified from monospecific antiserum by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The eluate was conjugated to biotin, re-purified, and then dialyzed against PBS with sodium azide prior to lyophilization.
Our Abpromise guarantee covers the use of ab24207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/3000.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 69 kDa.|
|ELISA||1/5000 - 1/25000.|
FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid.
- Information by UniProt
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
Anti-Human Serum Albumin antibody (Biotin) (ab24207) at 1/1000 dilution + Human Serum Albumin at 0.05 µg
HRP Streptavidin at 1/40000 dilution
Predicted band size: 69 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted?
ab24207 has been referenced in 1 publication.
- Tallman KA et al. Phospholipid-protein adducts of lipid peroxidation: synthesis and study of new biotinylated phosphatidylcholines. Chem Res Toxicol 20:227-34 (2007). PubMed: 17305406