• Product name

    Anti-Human Serum Albumin antibody (Biotin)
    See all Human Serum Albumin primary antibodies
  • Description

    Rabbit polyclonal to Human Serum Albumin (Biotin)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Human Serum Albumin.

  • Positive control

    • ICC/IF: HeLa cells.



Our Abpromise guarantee covers the use of ab24207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/3000.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
ELISA 1/5000 - 1/25000.


  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity

  • Involvement in disease

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • Analbuminemia antibody
    • Bisalbuminemia antibody
    • Cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • Dysalbuminemic hyperthyroxinemia antibody
    • Growth inhibiting protein 20 antibody
    • HSA antibody
    • Hyperthyroxinemia dysalbuminemic antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all


  • Anti-Human Serum Albumin antibody (Biotin) (ab24207) at 1/1000 dilution + Human Serum Albumin at 0.05 µg

    HRP Streptavidin at 1/40000 dilution

    Predicted band size: 69 kDa
    Observed band size: 61 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab24207 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24207 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Tallman KA  et al. Phospholipid-protein adducts of lipid peroxidation: synthesis and study of new biotinylated phosphatidylcholines. Chem Res Toxicol 20:227-34 (2007). Read more (PubMed: 17305406) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Thank you for your patience whilst I tried to find the information you requested.

I have talked to the lab and they have informed me that this polyclonal antibody was product against whole human serum albumin (HSA) and is tested against HSA by ELISA using Streptavidin HRP and TMBE for detection. By our testing a titer between 150K and 200K was obtained. This product also showed a single precipitin arc when tested by IEP against human serum, human serum albumin, anti biotin, and anti rabbit serum. The product was not tested against BSA.

I am sorry that I could not help anymore with this particular enquiry but if there is anything else I can help you with, please let me know.

Read More


Thank you for your reply.

I looked into all of the lot numbers that you provided and all of those lots actually came from the same master lot ie from the same batch. They were just aliquoted and put into stock at different times, hence the different lot numbers that were on the vials that you received.

I cannot find any information about any issues about decreased specificity or affinity for the different aliquots that you received.

If there is anything else I can help you with, or if you are experiencing any issues with the antibody that I could provide technical assistance with, then please let me know.

Read More


Thank you for contacting Abcam.

I have looked at the current stock of ab24207 and the only lot that we have is one that came into stock in June 2013 (lot# --). We do not have any other lots available.

I am sorry that I could not be of any more assistance in this matter, but please let me know if you have any further questions.

Read More
Human Purified protein (Sigma product, cat no A-1653)
Sigma product, cat no A-1653
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2

Abcam user community

Verified customer

Submitted Apr 11 2006


Thank you for your enquiry. Furhter to correspondence with the lab I have determined that BSA can be used as a blocking agent with this antibody. The conjugate itself is supplied with 10mg/mL of BSA as a stablizer. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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