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ab8941 is an HRP conjugate. Is the 'parental' antibody the ab8940 antibody? the spec/data sheet does not list a protein conc, what is the protein conc mg/ml IgG? Was there any clean up done after the conjugation reaction, are there aggregates of IgG or HRP present due to the conjugation protocol, or have these been removed? The buffer is listed as 0.15M Phosphate buffered saline pH 7.2. The 0.15M refers to the Phosphate concentration? The buffer is both 0.15M with respect to NaCl and Phosphate?
Asked on May 03 2005
Thank you for your enquiry. Yes this is the conjugated version of Ab8940. A standard conjugation protocol was employed (periodate method); which can be found on the internet. The aggregates of IgG or HRP were not removed as studies into this showed there to be no benefits. I would like to thank you for bringing the absences of a concentration value to my attention. The concentration of this product is 5mg/ml, I have updated the public facing data sheet in order to reflect this. The concentration of 0.15M refers to the NaCl concentration, which is generally 8.5g in 1L of solution.(I have checked the concentration using a standard recipe where: number of moles = mass/molar mass and concentration = number of moles/volume). If you have any further questions then please get back in touch with us.
Answered on May 10 2005