Key features and details
- Sensitivity: 130 pg/ml
- Range: 0.123 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Siglec 9 ELISA Kit (CD329)
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 130 pg/ml
Range0.123 ng/ml - 30 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.43 88% - 102% Serum 82.7 72% - 91% Plasma 78.16 70% - 91%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Siglec 9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Siglec 9 in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Siglec 9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Siglec 9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent D 1 x 15ml Biotinylated anti-Human Siglec 9 2 vials Recombinant Human Siglec 9 Standard (lyophilized) 2 vials Siglec 9 Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- SwissProt: Q9Y336 Human
ab113339 has not yet been referenced specifically in any publications.