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|Sample type||Average %||Range|
|Cell culture supernatant||101.3||91% - 108%|
|Serum||106.3||93% - 114%|
|Plasma||102.4||91% - 111%|
Abcam’s SIGLEC5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SIGLEC5 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human SIGLEC5 coated on a 96-well plate. Standards and samples are pipetted into the wells and SIGLEC5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SIGLEC5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human SIGLEC5||2 vials|
|Recombinant Human SIGLEC5 Standard (lyophilized)||2 vials|
|SIGLEC5 Microplate (12 x 8 wells)||1 unit|
|Stop Solution||1 x 25ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100641 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"