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    human-sirp-alpha-elisa-kitcd172a-ab272477.pdf

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Neuroscience Cell Adhesion Proteins ECM Proteins
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RecombinantSimpleStep

Human SIRP alpha ELISA Kit (CD172a)  (ab272477)

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Interpolated concentrations of native SIRP alpha in human U-937 cell extract.
  • Example of human SIRP alpha standard curve in 1X Cell Extraction Buffer PTR.
  • SimpleStep ELISA Protocol Diagram
  • Sandwich ELISA - Human SIRP alpha ELISA Kit (CD172a)  (ab272477)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 76.9 pg/ml
  • Range: 156.25 pg/ml - 10000 pg/ml
  • Sample type: Cell Lysate, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human SIRP alpha ELISA Kit (CD172a) 
    See all SIRP alpha kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    extract 8 2.5%
    Inter-assay
    Sample n Mean SD CV%
    extract 3 5.2%
  • Sample type

    Tissue Extracts, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    76.9 pg/ml
  • Range

    156.25 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 88 87% - 90%
    Cell Lysate 99 91% - 105%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human SIRP ELISA kit (ab272477) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SIRP protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SIRP with 76.90 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less


            -High sensitivity, specificity and reproducibility from superior antibodies


            -Fully validated in biological samples


            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    SIRP alpha (SIPRA or CD127a) is a 37.73 kD immunoglobulin-like cell surface receptor for CD47. SIRP alpha is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors, or insulin. SIRP alpha may play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity.) The antibodies are designed to a fragment of the extracellular domain of SIRP alpha.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human SIRP alpha (CD172a) Capture Antibody 1 x 600µl
    10X Human SIRP alpha (CD172a) Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human SIRP alpha (CD172a) Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
  • Function

    Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • Tissue specificity

    Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
  • Sequence similarities

    Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    N-glycosylated.
    Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P78324 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Signal regulatory protein alpha type 1
    • Bit
    • Brain Ig like molecule with tyrosine based activation motifs
    • Brain Ig-like molecule with tyrosine-based activation motifs
    • Brain immunoglobulin like molecule with tyrosine based activation motifs
    • CD172 antigen like family member A
    • CD172 antigen-like family member A
    • CD172a
    • CD172a antigen
    • Inhibitory receptor SHPS-1
    • Macrophage fusion receptor
    • MFR
    • MYD 1
    • Myd 1 antigen
    • MyD-1 antigen
    • p84
    • Protein tyrosine phosphatase non receptor type substrate 1
    • PTPNS1
    • SHP substrate 1
    • SHPS-1
    • SHPS1
    • SHPS1_HUMAN
    • Signal regulatory protein alpha
    • Signal regulatory protein alpha 2
    • Signal regulatory protein alpha 3
    • Signal regulatory protein alpha type 2
    • Signal-regulatory protein alpha-1
    • Signal-regulatory protein alpha-2
    • Signal-regulatory protein alpha-3
    • SIRP
    • Sirp-alpha-1
    • Sirp-alpha-2
    • Sirp-alpha-3
    • SIRPA
    • SIRPalpha
    • SIRPalpha1
    • SIRPalpha2
    • SIRPalpha3
    • Tyrosine phosphatase SHP substrate 1
    • Tyrosine protein phosphatase non receptor type substrate 1
    • Tyrosine-protein phosphatase non-receptor type substrate 1
    see all
  • Database links

    • Entrez Gene: 140885 Human
    • Omim: 602461 Human
    • SwissProt: P78324 Human
    • Unigene: 581021 Human

    Associated products

    • Alternative Versions

      • Human SIRP alpha Antibody Pair - BSA and Azide free (CD172a) (ab253364)

    Images

    • Interpolated concentrations of native SIRP alpha in human U-937 cell extract.
      Interpolated concentrations of native SIRP alpha in human U-937 cell extract.

      based on a 250 μg/mL extract load, SH-SY5Y cell extract based on a 2,000 μg/mL extract load, and brain tissue extract based on a 10 μg/mL extract load The concentrations of SIRP alpha were measured in duplicate and interpolated from the SIRP alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SIRP alpha concentration was determined to be 7.08 ng/mL in U-937 extract and 3.03 ng/mL in SH-SY5Y extract, and 6.85 ng/mL in brain extract.

    • Example of human SIRP alpha standard curve in 1X Cell Extraction Buffer PTR.
      Example of human SIRP alpha standard curve in 1X Cell Extraction Buffer PTR.
    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Sandwich ELISA - Human SIRP alpha ELISA Kit (CD172a)  (ab272477)
      Sandwich ELISA - Human SIRP alpha ELISA Kit (CD172a)  (ab272477)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab272477? Please let us know so that we can cite the reference in this datasheet.

    ab272477 has not yet been referenced specifically in any publications.

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