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Do you have a protocol for application "Coupling to beads via C-term Cys residues" What's the recommended conc. of the peptide?
Asked on Feb 03 2005
Thank you for your enquiry and your interest in our products. Here we are sending some useful websites which provide useful information for your customer. Peptide link & purification: http://www.perbio.com/link/link.asp?link=http://www.piercenet.com&tel=pierce Pull-down protocol: http://www.piercenet.com/Products/Browse.cfm?fldID=F3FD3612-415F-42A5-8922-736F9FDD36FB This product can be used as blocking peptide for Western blot application. The final concentration 0.1-1.0 ug/ml peptide should be pre-incubated with the antibody for around 20 min at room temperature before the blots are added. The recommended blocking buffer is 5% (w/v) BSA, 0.5% (v/v) Tween-20 in TBS or PBS as preferred. We hope this information is useful for the customer.
Answered on Feb 04 2005