Overview

  • Product name

    Human SIRT2 ELISA Kit
    See all SIRT2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 2.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.1%
  • Sample type

    Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 95 93% - 96%
    Cell culture extracts 104 93% - 116%
    Tissue Extracts 108 94% - 117%
    Heparin Plasma 84 82% - 86%
    EDTA Plasma 84 83% - 85%
    Citrate Plasma 83 80% - 84%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Product overview

    SIRT2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of SIRT2 protein in human cell and tissue extracts, serum, and plasma samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    SIRT2, or NAD-dependent protein deacetylase sirtuin-2, is an enzyme encoded by the SIRT2 gene. SIRT2 is a nicotinamide adenine dinucleotide (NAD+) dependent deacetylase which acts on the lysines on histones, alpha-tublin, and several transcription factors. SIRT2 functions as an intracellular regulatory protein and is involved in numerous cellular processes, such as cell cycle control, genomic integrity, microtubule dynamics, cell differentiation, metabolic networks, and autophagy. SIRT2 is expressed in a wide range of tissues and organs, with the highest expression observed in the brain.  


    Sensitivity:


    Samples diluted in 1X Cell Extraction Buffer PTR: 3 pg/mL.


    Samples diluted in Sample Diluent NS: 7 pg/mL.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human SIRT2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human SIRT2 Capture Antibody (Lyophilized) 1 vial
    Human SIRT2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • Tissue specificity

    Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • Sequence similarities

    Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • Developmental stage

    Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • Post-translational
    modifications

    Phosphorylated at the G2/M transition of the cell cycle.
  • Cellular localization

    Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • Alternative names

    • FLJ35621
    • FLJ37491
    • NAD dependent deacetylase sirtuin 2
    • NAD dependent protein deacetylase sirtuin 2
    • NAD-dependent deacetylase sirtuin-2
    • NAD-dependent protein deacetylase sirtuin-2
    • Regulatory protein SIR2 homolog 2
    • Silencing information regulator 2 like
    • Silent information regulator 2
    • SIR2
    • SIR2 like protein 2
    • Sir2 related protein type 2
    • SIR2, S. cerevisiae, homolog-loke 2
    • SIR2-like protein 2
    • SIR2L
    • SIR2L2
    • SIRT2
    • SIRT2_HUMAN
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae)
    • Sirtuin 2
    • Sirtuin type 2
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab227895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native SIRT2 based on a 150 µg/ml extract load in Human Fetal Brain Homogenate Extract, 600 µg/ml in U-87 MG Extract, 250 µg/ml in PC-3 Extract, and 125 µg/ml in THP-1 Extract. The concentrations of SIRT2 were measured in duplicate and interpolated from the SIRT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SIRT2 concentration was determined to be 998 pg/mL in Human Fetal Brain Homogenate Extract, 862 pg/mL in U-87 MG Extract, 978 pg/ml in PC-3 Extract, and 925 pg/ml in THP-1 Extract.

  • The concentrations of SIRT2 were measured in duplicates, interpolated from the SIRT2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab227895 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227895.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up