Human SOCS3 peptide (ab16199)

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Western blot - Human SOCS3 peptide (ab16199)

    Key features and details

    • Suitable for: WB, Blocking

    Description

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab16199 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      Blocking - Blocking peptide for Anti-SOCS3 antibody (ab16030)

    • Form

      Lyophilized

    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    • Reconstitution
      Reconstitute with 100 ul of deionised water (or equivalent).

    General Info

    • Alternative names

      • ATOD4
      • CIS 3
      • CIS-3
      • CIS3
      • Cish3
      • Cytokine induced SH2 protein 3
      • Cytokine-inducible SH2 protein 3
      • E2a Pbx1 target gene in fibroblasts 10
      • EF 10
      • MGC71791
      • SOCS 3
      • SOCS-3
      • Socs3
      • SOCS3_HUMAN
      • SSI 3
      • SSI-3
      • SSI3
      • STAT induced STAT inhibitor 3
      • STAT-induced STAT inhibitor 3
      • Suppressor of cytokine signaling 3
      see all

    • Function

      SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.

    • Tissue specificity

      Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

    • Pathway

      Protein modification; protein ubiquitination.

    • Involvement in disease

      Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.

    • Sequence similarities

      Contains 1 SH2 domain.
      Contains 1 SOCS box domain.

    • Domain

      The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
      The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.

    • Post-translational
      modifications

      Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
    • Information by UniProt

    Images

    • Western blot - Human SOCS3 peptide (ab16199)
      Western blot - Human SOCS3 peptide (ab16199)
      The 30kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SOCS3.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab16199? Please let us know so that we can cite the reference in this datasheet.

    ab16199 has not yet been referenced specifically in any publications.

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    Western blot Abreview for SOCS3 peptide

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    Abreviews
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    Application
    Western blot

    Sample: Human Cell lysate - whole cell (Jurkat cells)
    Loading amount: 10 µg
    Specification: Jurkat cells
    Treatment: see below (Additional notes)
    Gel Running Conditions: Reduced Denaturing (5-16% gels)
    Blocking step: BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Other product details
    Concentration: 1 µg/ml
    Incubation time: 23 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)

    Primary antibody (in addition to 'SOCS3 peptide')
    Concentration: 1 µg/ml

    Secondary antibody
    Name: Non-Abcam antibody was used: anti-rabbit IgG
    Host species: Horse
    Conjugation: Horse Radish Peroxidase
    Dilution: 1/2000

    Detection
    Detection method: chemiluminescence
    Exposure: 45 minute(s) and 0 second(s)
    Bands: Specific: 25 kDa
    Positive control: Jurkat cells non stimulated 30min
    Jurkat cells CD3/28 stimulated 30min

    Additional data
    Additional Notes: Lanes:
    1 = negative control / loading control (GAPDH protein)
    2 = Jurkat cells non stimulated 30min
    3 = Jurkat cells CD3/28 stimulated 30min
    4 = SOCS 3 peptide (ab16199) 1,0µg/ml
    5 = SOCS 3 peptide (ab16199) 0,5µg/ml
    6 = SOCS 3 peptide (not abcam) 1µg/ml
    7 = SOCS 3 peptide (not abcam) 0,5µg/ml

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    Verified customer

    Submitted Jan 06 2012

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