Overview

  • Product name

    Human Soluble TNF Receptor I ELISA Kit
    See all TNF Receptor I kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 1 pg/ml
  • Range

    0.55 pg/ml - 400 pg/ml
  • Recovery

    80 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 80.02 71% - 90%
    Serum 80.43 72% - 91%
    Plasma 81.36 73% - 91%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human STNF-R1 (soluble tumor necrosis factor receptor I) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human STNF-R1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human STNF-R1 coated on a 96-well plate. Standards and samples are pipetted into the wells and STNF-R1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human STNF-R1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STNF-R1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100642

Protocols

References

This product has been referenced in:

  • Strøm Halden TA  et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014). Read more (PubMed: 24452849) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

It is not known whether this kit will detect the TNF-sTNFR1 complex or not. We know it will detect free soluble TNF R1 and it does not cross react with TNF-alpha, TNF-beta or soluble TNF RII. Whether though the kit will detect soluble TNF R1 complexed with one of these ligands though has not been tested.

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Answer

Thank you for your enquiry regarding these kits.

I would suggest reading through the Protocol Booklets for each of these kits provided on the product datasheets. These documents contain specific information about the sample types, sample preparations, protocol, instruments and equipment required for the assays. Whole blood samples are not used to test these kits mainly serum or plasma instead.

ab46044: https://www.abcam.com/high-sensitivity-il6-human-elisa-kit-2-x-96-well-plates-ab46044.html

ab100642: https://www.abcam.com/ps/products/100/ab100642/documents/ab100642-Soluble-TNF-Receptor-I-Human-ELISA-Kit-ab100642-protocol-plain.pdf

ab100643: https://www.abcam.com/ps/products/100/ab100643/documents/ab100643-Soluble-TNF-Receptor-II-Human-ELISA-Kit-ab100643-protocol-plain.pdf

ab108826: https://www.abcam.com/ps/products/108/ab108826/documents/ab108826%20C%20Reactive%20Protein%20Human%20ELISA_v2%20(Website).pdf

As you are aware our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. Therefore please advsie yiur customer to use teh product as soon as possible after purchase to make sure that we can provide the best technical support within the guarantee period.

The specific storage instructions can also be found on the product datasheets. It is not clear if the customer would like to use these products in serum from normal, or drug treated or diseased (pathological conditions) individuals. We would suggest optimizing the assay and using standards.

Let me know if you haveany further questions.

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