Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.55 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Soluble TNF Receptor I ELISA Kit
See all TNF Receptor I kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 1 pg/ml
Range0.55 pg/ml - 400 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 80.02 71% - 90% Serum 80.43 72% - 91% Plasma 81.36 73% - 91%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Human STNF-R1 (soluble tumor necrosis factor receptor I) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human STNF-R1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human STNF-R1 coated on a 96-well plate. Standards and samples are pipetted into the wells and STNF-R1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human STNF-R1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STNF-R1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human Soluble TNF Receptor I (lyophilized) 2 vials Recombinant Human Soluble TNF Receptor I Standard (lyophilized) 2 vials Soluble TNF Receptor I Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Involvement in diseaseFamilial hibernian fever
Multiple sclerosis 5
Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats.
DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
modificationsThe soluble form is produced from the membrane form by proteolytic processing.
Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
- Information by UniProt
ab100642 has been referenced in 1 publication.
- Strøm Halden TA et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014). PubMed: 24452849