Overview

  • Product name

    Human soluble TREM1 ELISA Kit
    See all TREM1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 7.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 10.4%
  • Sample type

    Cell culture supernatant
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    154 pg/ml
  • Range

    500 pg/ml - 32000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 120 118% - 123%
    Cell culture media 90 85% - 100%
    Heparin Plasma 115 113% - 118%
    EDTA Plasma 118 113% - 121%
    Citrate Plasma 110 105% - 115%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    soluble TREM1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble TREM1 protein in cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples diluted in Sample Diluent NS - 154 pg/mL
    Samples diluted in Sample Diluent 25BS - 161 pg/mL

  • Notes

    The triggering receptor expressed on myeloid cells (TREM), modulate the innate response either by amplifying or dampening Toll-Like Receptor (TLR) induced signals, and act to fine-tuning the inflammatory response. Trem-1 is a member of the TREM family of receptors encoded by a cluster of genes localized to the Human chromosome 6p21.1. The presence of TREM and TREM-like receptors are expressed on a variety of innate cells of the myeloid lineage including neutrophils, monocytes, macrophages, microglia, dendritic cells, among others. Soluble Trem-1 is thought to arise through the cleavage of the receptor by metalloproteinases. Where it acts to negatively regulate TREM receptor signaling through neutralization of the respective ligands and minimize tissue damage due to prolonged inflammatory response.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human sTREM1 Capture Antibody 1 x 600µl
    10X Human sTREM1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human sTREM1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    TREM1 is also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. TREM1 is a Type I membrane protein that contains an immunoglobulin-like V-type domain. An alternatively spliced protein variant may be secreted. TREM1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock.
  • Cellular localization

    Secreted and Cell membrane.
  • Alternative names

    • CD354
    • TREM 1
    • Triggering receptor expressed on monocytes 1
    • Triggering receptor expressed on myeloid cells 1
    • Triggering receptor TREM 1
    • Triggering receptor TREM1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab185987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • HL60 cells were grown in culture with or without the addition of (5 µg/mL) LPS for 24 hours before supernatants were collected and assayed. Background subtracted data from duplicate measurements are plotted.

  • HL60 cells were grown in culture with or without the addition of (5 µg/mL) LPS for 24 hours before supernatants were collected and assayed. Bar graph denotes quantification of sTREM1 interpolated from standard curve and multiplied by dilution factor.

Protocols

References

ab185987 has not yet been referenced specifically in any publications.

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