• Product name
    Human Sonic Hedgehog ELISA Kit
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 91.72 82% - 101%
    Serum 95.34 84% - 103%
    Plasma 76.43 68% - 89%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Sonic Hedgehog Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Sonic Hedgehog in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human Sonic Hedgehog coated on a 96-well plate. Standards and samples are pipetted into the wells and Sonic Hedgehog present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Sonic Hedgehog antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Sonic Hedgehog bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Human Sonic Hedgehog (lyophilized) 2 units
    Recombinant Human Sonic Hedgehog Standard (lyophilized) 2 vials
    Sonic Hedgehog Microplate (12 x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Sonic Hedgehog, which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
  • Alternative names
    • HHG 1
    • HHG1
    • HLP3
    • HPE3
    • MCOPCB5
    • SHH
    • SMMCI
    • Sonic hedgehog homolog
    • Sonic hedgehog protein
    • Sonic hedgehog protein C product
    • Sonic hedgehog protein N product
    • TPT
    • TPTPS
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100639

  • Representative standard curve using ab100639



This product has been referenced in:
  • Estep M  et al. Hepatic sonic hedgehog protein expression measured by computer assisted morphometry significantly correlates with features of non-alcoholic steatohepatitis. BMC Gastroenterol 19:27 (2019). Read more (PubMed: 30744560) »
  • Ihnatovych I  et al. Timing of Wnt Inhibition Modulates Directed Differentiation of Medial Ganglionic Eminence Progenitors from Human Pluripotent Stem Cells. Stem Cells Int 2018:3983090 (2018). Read more (PubMed: 30050576) »
See all 12 Publications for this product

Customer reviews and Q&As

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We tested this product on pig brain tissue (thawed from snapfrozen in liquid nitrogen), since we were informed that there is a 95% homology between human and pig SHH protein sequences. Since no information was available for which diluent to use, we found diluent B most appropiate and used this. We followed the protocol step by step.
We had no problem getting acceptable results from the ELISA and we will now proceed to order yet another kit for the remaining brain samples to be analyzed.
Although the kit worked fine, the manual is somewhat messy at places (e.g. the peculiar dilutions used in the standard curve could use some explanation), but nevertheless, the kit seems to work fine in pig brain tissue.

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Submitted Nov 21 2013

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