Overview

  • Product name
    Human Sorbitol Dehydrogenase ELISA Kit
    See all Sorbitol Dehydrogenase kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Jurkat cells 5 2.3%
    Inter-assay
    Sample n Mean SD CV%
    Jurkat cells 3 7.9%
  • Sample type
    Urine, Serum, Cell culture extracts, Tissue Extracts, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    60 pg/ml
  • Range
    0.39 ng/ml - 25 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 107 102% - 113%
    Serum 95 92% - 97%
    Cell culture extracts 99 89% - 109%
    Tissue Culture Media 103 92% - 114%
    Citrate Plasma 86 81% - 90%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Sorbitol Dehydrogenase in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Sorbitol Dehydrogenase protein in human serum, plasma, urine, cell and tissue extract.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sorbitol Dehydrogenase is an enzyme that converts sorbitol to fructose and is located mostly in the liver and to some extent in the kidney and testes. Elevated levels of Sorbitol Dehydrogenase is a biomarker for hepatocellular injury.  Also tissues that normally have low levels of sorbitol dehydrogenase can accumulate sorbitol dehydrogenase under conditions of hyperglycemia.


     

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab233613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • The Sorbitol Dehydrogenase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The Sorbitol Dehydrogenase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Sorbitol Dehydrogenase were measured in duplicates, interpolated from the Sorbitol Dehydrogenase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 25%, and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of Sorbitol Dehydrogenase were measured in duplicates, interpolated from the Sorbitol Dehydrogenase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Sorbitol Dehydrogenase concentration was determined to be 19 ng/mL in serum and 16 ng/mL in plasma(citrate).

  • Interpolated concentrations of native Sorbitol Dehydrogenase in human Jurkat cell extract and human liver tissue extract samples.

  • The concentrations of Sorbitol Dehydrogenase were measured in duplicate and interpolated from the Sorbitol Dehydrogenase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Sorbitol Dehydrogenase concentration was determined to be 0.06 ng/µg in Jurkat cell extract and 3.3 ng/µg in liver tissue extract.

Protocols

References

ab233613 has not yet been referenced specifically in any publications.

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