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    human-sost-elisa-kit-ab155440.pdf

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Signal Transduction Growth Factors/Hormones TGF
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Human SOST ELISA Kit (ab155440)

  • Datasheet
  • SDS
  • Protocol Booklet
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SOST Human ELISA Kit

    Key features and details

    • Sensitivity: 15 pg/ml
    • Range: 0.017 ng/ml - 10 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Product image
    Recombinant human Sclerostin protein (Active) (ab167702)

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    Overview

    • Product name

      Human SOST ELISA Kit
      See all SOST kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 12%
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 15 pg/ml
    • Range

      0.017 ng/ml - 10 ng/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum 123.3 110% - 136%
      Plasma 125.7 118% - 134%
      Cell culture media 96.04 86% - 105%
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s SOST Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SOST in serum, plasma and cell culture supernatants.

      This assay employs an antibody specific for Human SOST coated on a 96-well plate. Standards and samples are pipetted into the wells and SOST present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SOST antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SOST bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • Notes

      Optimization may be required with urine samples.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer Concentrate 1 x 25ml
      5X Assay Diluent 1 x 15ml
      500X HRP-Streptavidin Concentrate 1 x 200µl
      Biotinylated anti-Human SOST 2 vials
      Recombinant Human SOST Standard (lyophilized) 2 vials
      SOST Microplate (12 x 8 wells) 1 unit
      Stop Solution 1 x 8ml
      TMB One-Step Substrate Reagent 1 x 12ml
    • Research areas

      • Signal Transduction
      • Growth Factors/Hormones
      • TGF
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • Structures
      • Bone
      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Secreted
      • Developmental Biology
      • Organogenesis
      • Skeletal development
      • Bone
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Signal transduction proteins ELISA kits
      • Metabolism
      • Types of disease
      • Metabolic disorders
    • Relevance

      Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
    • Cellular localization

      Secreted; extracellular space; extracellular matrix
    • Alternative names

      • Sclerostin
    • Database links

      • Entrez Gene: 50964 Human
      • Omim: 605740 Human
      • SwissProt: Q9BQB4 Human

      Associated products

        Images

        • SOST Human ELISA Kit
          SOST Human ELISA Kit

          Representative standard curve using ab155440

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (0)

        Publishing research using ab155440? Please let us know so that we can cite the reference in this datasheet.

        ab155440 has not yet been referenced specifically in any publications.

        Customer reviews and Q&As

        Show All Reviews Q&A
        Submit a review Submit a question

        Question

        1) assay diluent 5x?
        dilution 5-fold = 1:4 or 1:5?
        2) Ab concentrate, storage 4 degree for 5 days. Can it be stored at -20 degree for longer?

        Read More

        Abcam community

        Verified customer

        Asked on May 17 2013

        Answer


        To answer your questions by email as well:

        1) The assay diluent is provided as 5x (dilution is 1 part:4 parts).


        2) The antibody concentrate is not recommended to be stored at -20 degree. It can be stored at 4 degree a bit longer than the recommended 5 days if necessary, but this was not further tested.

        Read More

        Abcam Scientific Support

        Answered on May 17 2013

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
        For licensing inquiries, please contact partnerships@abcam.com

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