Human SOST ELISA Kit (ab155440)

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SOST Human ELISA Kit

    Key features and details

    • Sensitivity: 15 pg/ml
    • Range: 0.017 ng/ml - 10 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human SOST ELISA Kit
      See all SOST kits

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 12%

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 15 pg/ml

    • Range

      0.017 ng/ml - 10 ng/ml

    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum 123.3 110% - 136%
      Plasma 125.7 118% - 134%
      Cell culture media 96.04 86% - 105%

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s SOST Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SOST in serum, plasma and cell culture supernatants.

      This assay employs an antibody specific for Human SOST coated on a 96-well plate. Standards and samples are pipetted into the wells and SOST present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SOST antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SOST bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • Notes

      Optimization may be required with urine samples.

    • Platform

      Microplate

    Properties

    Associated products

      Images

      • SOST Human ELISA Kit
        SOST Human ELISA Kit

        Representative standard curve using ab155440

      References (0)

      Publishing research using ab155440? Please let us know so that we can cite the reference in this datasheet.

      ab155440 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Question

      1) assay diluent 5x?
      dilution 5-fold = 1:4 or 1:5?
      2) Ab concentrate, storage 4 degree for 5 days. Can it be stored at -20 degree for longer?

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      Answer


      To answer your questions by email as well:

      1) The assay diluent is provided as 5x (dilution is 1 part:4 parts).


      2) The antibody concentrate is not recommended to be stored at -20 degree. It can be stored at 4 degree a bit longer than the recommended 5 days if necessary, but this was not further tested.

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