Human SOST ELISA Kit (ab155440)

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Overview

  • Product name

    Human SOST ELISA Kit
    See all SOST kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 15 pg/ml

  • Range

    0.017 ng/ml - 10 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 123.3 110% - 136%
    Plasma 125.7 118% - 134%
    Cell culture media 96.04 86% - 105%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s SOST Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SOST in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human SOST coated on a 96-well plate. Standards and samples are pipetted into the wells and SOST present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SOST antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SOST bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SOST Human ELISA Kit
    SOST Human ELISA Kit

    Representative standard curve using ab155440

Protocols

References

ab155440 has not yet been referenced specifically in any publications.

Publishing research using ab155440? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

1) assay diluent 5x?
dilution 5-fold = 1:4 or 1:5?
2) Ab concentrate, storage 4 degree for 5 days. Can it be stored at -20 degree for longer?

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Answer


To answer your questions by email as well:

1) The assay diluent is provided as 5x (dilution is 1 part:4 parts).


2) The antibody concentrate is not recommended to be stored at -20 degree. It can be stored at 4 degree a bit longer than the recommended 5 days if necessary, but this was not further tested.

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