Human SOST ELISA Kit (ab155440)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 0.017 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SOST ELISA Kit
See all SOST kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 15 pg/ml -
Range
0.017 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 123.3 110% - 136% Plasma 125.7 118% - 134% Cell culture media 96.04 86% - 105% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s SOST Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SOST in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human SOST coated on a 96-well plate. Standards and samples are pipetted into the wells and SOST present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SOST antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SOST bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-Human SOST 2 vials Recombinant Human SOST Standard (lyophilized) 2 vials SOST Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. -
Cellular localization
Secreted; extracellular space; extracellular matrix -
Alternative names
- Sclerostin
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Database links
- Entrez Gene: 50964 Human
- Omim: 605740 Human
- SwissProt: Q9BQB4 Human
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155440 has been referenced in 1 publication.
- Zheng S et al. Sclerostin aggravates cardiac remodeling after myocardial infarction by inhibition of Wnt/β-catenin signaling pathway. J Thorac Dis 14:1563-1577 (2022). PubMed: 35693595