For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    human-sost-elisa-kit-ab221836.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Stem Cells Signaling Pathways Wnt Secreted
Share by email
RecombinantSimpleStep

Human SOST ELISA Kit (ab221836)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Other - Human SOST ELISA Kit (ab221836)
  • Human SOST standard curve
  • Interpolated concentrations of spike SOST in human serum, plasma and cell culture supernatant samples
  • Serum from ten individual healthy human female donors was measured in duplicate
  • Interpolated concentrations of native SOST in human serum and plasma samples
  • Sandwich ELISA - Human SOST ELISA Kit (ab221836)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 6 pg/ml
  • Range: 31.1 pg/ml - 2000 pg/ml
  • Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

You may also be interested in

ELISA
Product image
Human IL-6 ELISA Kit (ab100572)
Protein
Product image
Recombinant human Sclerostin protein (Active) (ab167702)

View more associated products

Overview

  • Product name

    Human SOST ELISA Kit
    See all Sclerostin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 8.6%
  • Sample type

    Serum, Cell culture media, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    6 pg/ml
  • Range

    31.1 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 106 104% - 109%
    Cell culture media 117 113% - 121%
    EDTA Plasma 97 95% - 99%
    Cit plasma 108 104% - 114%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Cow
  • Product overview

    Human SOST ELISA Kit (ab221836) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SOST protein in cell culture media, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SOST with 6 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Sclerostin is a secreted protein important in regulation of bone growth.  Sclerostin (SOST) is widely expressed and can be found at higher levels in bone, cartilage, kidney, liver and bone marrow.  Mutations in the SOST gene can lead to various diseases including Van Buchem disease (VBCH) and Craniodiaphyseal dysplasia autosomal dominant (CDD)).

  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human SOST Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human SOST Capture Antibody (lyophilized) 1 vial
    Human SOST Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted
  • Function

    Negative regulator of bone growth.
  • Tissue specificity

    Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days.
  • Involvement in disease

    Defects in SOST are the cause of sclerosteosis (SOST) [MIM:269500]; also known as cortical hyperostosis with syndactyly. SOST is an autosomal recessive sclerosing bone dysplasia characterized by a generalized hyperostosis and sclerosis leading to a markedly thickened skull, with mandible, ribs, clavicles and all long bones also being affected. Due to narrowing of the foramina of the cranial nerves, facial nerve palsy, hearing loss and atrophy of the optic nerves can occur. Sclerosteosis is clinically and radiologically very similar to van Buchem disease, mainly differentiated by hand malformations and a large stature in sclerosteosis patients.
    Note=A 52 kb deletion downstream of SOST results in SOST transcription suppression and is a cause of van Buchem disease (VBCH) [MIM:239100]; also known as hyperostosis corticalis generalisata. VBCH is an autosomal recessive sclerosing bone dysplasia characterized by endosteal hyperostosis of the mandible, skull, ribs, clavicles, and diaphyses of the long bones. Affected patients present a symmetrically increased thickness of bones, most frequently found as an enlarged jawbone, but also an enlargement of the skull, ribs, diaphysis of long bones, as well as tubular bones of hands and feet. The clinical consequence of increased thickness of the skull include facial nerve palsy causing hearing loss, visual problems, neurological pain, and, very rarely, blindness as a consequence of optic atrophy. Serum alkaline phosphatase levels are elevated.
  • Sequence similarities

    Belongs to the sclerostin family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q9BQB4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • BEER
    • CDD
    • Cortical hyperostosis with syndactyly
    • Sclerosteosis
    • Sclerostin
    • Sost
    • SOST_HUMAN
    • SOST1
    • UNQ2976/PRO7455/PRO7476
    • VBCH
    see all
  • Database links

    • Entrez Gene: 50964 Human
    • Omim: 605740 Human
    • SwissProt: Q9BQB4 Human
    • Unigene: 349204 Human

    Associated products

    • Alternative Versions

      • Human SOST Antibody Pair - BSA and Azide free (ab241918)

    Images

    • Other - Human SOST ELISA Kit (ab221836)
      Other - Human SOST ELISA Kit (ab221836)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Human SOST standard curve
      Human SOST standard curve

      Example of human SOST standard curve. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spike SOST in human serum, plasma and cell culture supernatant samples
      Interpolated concentrations of spike SOST in human serum, plasma and cell culture supernatant samples

      Interpolated concentrations of spiked SOST in human serum, plasma and cell culture supernatant samples. The concentrations of SOST were measured in duplicates, interpolated from the SOST standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Serum from ten individual healthy human female donors was measured in duplicate
      Serum from ten individual healthy human female donors was measured in duplicate

      Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SOST concentration was determined to be 552 pg/mL with a range of 331 – 781 pg/mL.

    • Interpolated concentrations of native SOST in human serum and plasma samples
      Interpolated concentrations of native SOST in human serum and plasma samples

      Interpolated concentrations of native SOST in human serum and plasma samples. The concentrations of SOST were measured in duplicates, interpolated from the SOST standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SOST concentration was determined to be 452.2 pg/mL in serum, 657 pg/mL in plasma (citrate) and 302 pg/mL in plasma (EDTA).

       

    • Sandwich ELISA - Human SOST ELISA Kit (ab221836)
      Sandwich ELISA - Human SOST ELISA Kit (ab221836)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab221836? Please let us know so that we can cite the reference in this datasheet.

    ab221836 has been referenced in 1 publication.

    • Zhou F  et al. Decreased Sclerostin Secretion in Humans and Mice With Nonalcoholic Fatty Liver Disease. Front Endocrinol (Lausanne) 12:707505 (2021). PubMed: 34421825

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab221836.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.