Overview

  • Product name

    Human SPARCL1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 269pg/ml 11.03 4.1%
    2 16 1195pg/ml 57.36 4.8%
    3 16 2030pg/ml 105.6 5.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 321pg/ml 18.3 5.7%
    2 24 1254pg/ml 79 6.3%
    3 24 2208pg/ml 167.8 7.6%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    46.9 pg/ml - 3000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human SPARCL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213826) is designed for the quantitative measurement of Human SPARCL1 in cell culture supernatants and serum.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for SPARCL1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: I17-F66) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPARCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SPARCL1 amount of sample captured in plate.

  • Notes

    SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of non-lymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human SPARCL1 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human SPARCL1 antibody 1 x 130µl
    Lyophilized recombinant Human SPARCL1 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Tissue specificity

    Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • Post-translational
    modifications

    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Thr-398 is the preferred site to Thr-399.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names

    • Ecm2
    • Extracellular matrix protein 2
    • Hevin
    • High endothelial venule protein
    • MAST 9
    • mast9
    • Matrix glycoprotein Sc1
    • PIG33
    • proliferation inducing protein 33
    • RAGS1 antigen
    • SC1
    • SPARC like 1
    • SPARC-like protein 1
    • Sparcl1
    • SPRL1_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human SPARCL1 ELISA Kit (ab213826) Standard Curve.

Protocols

References

ab213826 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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