Overview

  • Product name

    Human Stanniocalcin 1 / STC ELISA Kit (STC1)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 246pg/ml 14.02 5.7%
    2 16 801pg/ml 48.06 6%
    3 16 1351pg/ml 74.3 5.5%
    Inter-assay
    Sample n Mean SD CV%
    1 24 254pg/ml 15.74 6.2%
    2 24 775pg/ml 49.6 6.4%
    3 24 1416pg/ml 67.96 6.8%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    31.2 pg/ml - 2000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Stanniocalcin 1 / STC Enzyme-Linked Immunosorbent Assay (ELISA) kit (STC1) (ab213829) is designed for the quantitative measurement of Human Stanniocalcin 1 / STC in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Stanniocalcin 1 / STC has been pre-coated onto 96-well plates. Standards (NSO, V34-A247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Stanniocalcin 1 / STC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Stanniocalcin 1 / STC amount of sample captured in plate.

  • Notes

    Stanniocalcin 1 / STC is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin 1 / STC is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. Stanniocalcin 1 / STC interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of Stanniocalcin 1 / STC is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies show that Stanniocalcin 1 / STC is an anti-parallel homodimer in solution and the cysteine 202 is responsible for the dimerization of this protein. All the 5 disulfide bonds of human STC1 are conserved and have the same profile of fish STC.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Stanniocalcin 1 / STC ELISA Kit (STC1) (ab213829) Standard Curve.

Protocols

References

ab213829 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213829.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up