Overview

  • Product name

    Human Stanniocalcin 2 / STC-2 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 5.2%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 9.6%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.95 ng/ml
  • Range

    1.25 ng/ml - 80 ng/ml
  • Recovery

    96 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Rabbit, Cow, Dog
  • Product overview

    The Human Stanniocalcin 2 / STC-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222880) is designed for detection of Stanniocalcin 2 / STC-2 in human plasma, serum, and cell culture samples.


    This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Stanniocalcin 2 / STC-2 in approximately 4 hours. A polyclonal antibody specific for human Stanniocalcin 2 / STC-2 has been pre-coated onto a 96-well microplate with removable strips. Stanniocalcin 2 / STC-2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human Stanniocalcin 2 / STC-2, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Stanniocalcin 2 / STC-2, also known as stanniocalcin-related protein, is a secreted homodimeric glycoprotein that belongs to the stanniocalcin family. Stanniocalcin 2 / STC-2 contains 302 amino acid residues with 34% STC-1 identity and a molecular mass of 32 kDa. The N terminus of Stanniocalcin 2 / STC-2 contains a signal peptide followed by a cysteine-rich region that may mediate homodimerization. In the kidney, the function of Stanniocalcin 2 / STC-2 on phosphate transport appears to be the opposite to that of STC-1. Stanniocalcin 2 / STC-2 is phosphorylated by casein kinase-2 on serine residues and can inhibit phosphorylation of cell-surface proteins. It is expressed in a wide variety of tissues, including the pancreas, skeletal muscle, and small intestine. Stanniocalcin 2 / STC-2 may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, and cellular calcium/phosphate homeostasis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Standard Diluent 1 x 2ml
    20X Wash Buffer Concentrate 2 x 30ml
    Anti- Human Stanniocalcin-2 / STC-2 coated Microplate (12 x 8 wells) 1 unit
    Biotinylated Human Stanniocalcin-2 / STC-2 antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Human Stanniocalcin-2 / STC-2 Standard 1 vial
    Sealing Tapes 1 x 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Has an anti-hypocalcemic action on calcium and phosphate homeostasis.
  • Tissue specificity

    Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the stanniocalcin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Stanniocalcin 2
    • Stanniocalcin related protein
    • Stanniocalcin-2
    • Stanniocalcin-related protein
    • Stanniocalcin2
    • STC 2
    • STC related protein
    • STC-2
    • STC-related protein
    • STC2
    • STC2_HUMAN
    • STCRP
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of human Stanniocalcin 2 / STC-2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab222880 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222880.
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