For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    human-stat5-tyr694-in-cell-elisa-kit-ab126429.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Signaling Pathway Nuclear Signaling STATs
Share by email

Human STAT5 (Tyr694) In-Cell ELISA Kit (ab126429)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

In-Cell ELISA - STAT5 (Tyr694) Human In-Cell ELISA Kit (ab126429)
  • In-Cell ELISA - STAT5 (Tyr694) Human In-Cell ELISA Kit (ab126429)
  • Western blot - STAT5 (Tyr694) Human In-Cell ELISA Kit (ab126429)

Key features and details

  • Assay type: Semi-quantitative
  • Detection method: Colorimetric
  • Sample type: Adherent cells
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human STAT5a protein (ab84627)

View more associated products

Overview

  • Product name

    Human STAT5 (Tyr694) In-Cell ELISA Kit
    See all STAT5 kits
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells
  • Assay type

    Semi-quantitative
  • Assay time

    5h 10m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab126429 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of STAT5 (Tyr694) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human cell lines. By determining STAT5 protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.


    In the In-Cell STAT5 (Tyr694) ELISA kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti Phospho-STAT5 (Tyr694) or Anti-STAT5 (primary antibody) is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-mouse IgG (secondary antibody) is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    HRP-conjugated Anti-Mouse IgG Concentrate 1 x 10µl
    Blocking Buffer Concentrate (5X) 1 x 20ml
    Fixing Solution 1 x 30ml
    Uncoated 96-well Microplate 1 unit
    Mouse Anti-Phospho-STAT5 (Tyr694) Concentrate (Item G) 1 x 7µl
    Mouse Anti-STAT5 Concentrate (Item H) 1 x 10µl
    Quenching Buffer Concentrate (30x) 1 x 2ml
    Stop Solution 1 x 14ml
    TMB One-Step Substrate Reagent 1 x 12ml
    Wash Buffer A Concentrate (20X) 1 x 30ml
    Wash Buffer B Concentrate (20X) 1 x 30ml
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • STAT family
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • In Cell ELISA Kits
    • Phosphoprotein ICE kits
  • Function

    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Target information above from: UniProt accession P42229 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • MGF
    • Signal transducer and activator of transcription 5A
    • Signal Transducer and Activator of Transcription 5B
    • STA5A_HUMAN
    • STAT 5A
    • STAT 5B
    • STAT5
    • STAT5A
    • STAT5B
    • Transcription factor STAT5A
    • Transcription factor STAT5B
    see all
  • Database links

    • Entrez Gene: 6777 Human
    • Entrez Gene: 6776 Human
    • Omim: 601511 Human
    • Omim: 604260 Human
    • SwissProt: P51692 Human
    • SwissProt: P42229 Human
    • Unigene: 437058 Human

    Associated products

      Images

      • In-Cell ELISA - STAT5 (Tyr694) Human In-Cell ELISA Kit (ab126429)
        In-Cell ELISA - STAT5 (Tyr694) Human In-Cell ELISA Kit (ab126429)

        A431 cells were stimulated by different concentrations of EGF for 10 minutes at 37°C.

         

      • In-Cell ELISA - STAT5 (Tyr694) Human In-Cell ELISA Kit (ab126429)
        In-Cell ELISA - STAT5 (Tyr694) Human In-Cell ELISA Kit (ab126429)

        A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37°C.

      • Western blot - STAT5 (Tyr694) Human In-Cell ELISA Kit (ab126429)
        Western blot - STAT5 (Tyr694) Human In-Cell ELISA Kit (ab126429)
        Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells. Phospho-STAT5 (Tyr694) and STAT5 antibodies were used in both detection assays.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab126429? Please let us know so that we can cite the reference in this datasheet.

      ab126429 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Can I use the ab126429 kit also for analysis of STAT5 phosphorylation of human PBMC (non-adherent cells)? If the answer is yes, how should I modify the protocol?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 19 2014

      Answer

      We have not tried the assay with non-adherent cells and we are not aware of any other lab trying this either. It might be accomplished by centrifuging the cells in a 96-well plate after each wash, to pull them down into pellets in each well, but you would likely have some cell loss during aspiration of the washes, and possibly some loss due to cell lysis from the centrifugation. You could check to see how much by testing centrifugation of your cells in a plate (assuming you have plate holders) a few times, without actually doing the assay.

      Read More

      Tom Ruyle

      Abcam Scientific Support

      Answered on Aug 19 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.