Human Superoxide Dismutase 1 ELISA Kit (ab119520)

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Overview

  • Product name

    Human Superoxide Dismutase 1 ELISA Kit
    See all Superoxide Dismutase 1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5.8%

  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.04 ng/ml

  • Range

    0.08 ng/ml - 5 ng/ml

  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Serum 98 89% - 108%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s Human Superoxide Dismutase 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cu/Zn Superoxide Dismutase concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, fetal umbilical vein blood and urine.


    Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.

  • Notes

    ab119520 Human Superoxide Dismutase 1 ELISA kit has been shown to detect both SOD1 and SOD3.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab119520.

Protocols

References

This product has been referenced in:

  • Stradling JR  et al. Biomarkers of oxidative stress following continuous positive airway pressure withdrawal: data from two randomised trials. Eur Respir J 46:1065-71 (2015). Sandwich ELISA ; Human . Read more (PubMed: 26022961) »
See 1 Publication for this product

Publishing research using ab119520? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Could this kit recognize the G93A mutant protein, mutated at residue 93?
How should cell culture supernatants be prepared? There are specific instructions about how to prepare other sample types.

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Answer

Thank you for your call last week and for your patience while I have been in touch with the lab about your enquiry.

Theepitopse of the antibodies used in the kit have not been mapped at this point, and the kit has never been tested with samples containing the G93A mutant protein, so unfortunately we do not know for sure if the kit will work with these samples.

For cell culture supernatants, there isn't a particular recommended dilution because the concentration of the analyte can vary so much between these samples. We suggest trying several dilutions in order to find the optimal dilution for your particular samples.

I am sorry thatwe don't have further information, but please let me know if you have any other questions or if there is anything else that we can do for you, and I'll be happy to help.

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Question

Hello, I would like to find out if you would sell the antibody pair (un-conjugated) and protein standard for human Cu/Zn Superoxide Dismutase. I would like to purchase enough reagents for ~5 plates for a proof-of-concept expt.

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Answer

Thank you for contacting us with your enquiry. I have looked through our catalogue and have found two sets that may be of interest to use. ab88404: https://www.abcam.com/Superoxide-Dismutase-1-antibody-ab88404.html and it's peptide ab99369: https://www.abcam.com/Superoxide-Dismutase-1-peptide-ab99369.html Another set that may interest you are: ab45777 : https://www.abcam.com/Superoxide-Dismutase-1-antibody-ab45777.html the related peptide is ab46993: https://www.abcam.com/Superoxide-Dismutase-1-peptide-ab46993.html We also offer a Superoxide Dismutase Activity Colorimetric Assay Kit (ab65354) and a Cu/Zn Superoxide Dismutase Human ELISA kit (ab119520). I hope this information is helpful. Please contact us if you have any further questions.

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