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    human-superoxide-dismutase-1-elisa-kit-ab119520.pdf

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Cell Biology Other Antibodies Oxidative Stress
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Human Superoxide Dismutase 1 ELISA Kit (ab119520)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (5)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.04 ng/ml
    • Range: 0.08 ng/ml - 5 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human Superoxide Dismutase 1 ELISA Kit
      See all Superoxide Dismutase 1 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 5.1%
      Inter-assay
      Sample n Mean SD CV%
      Overall 5.8%
    • Sample type

      Cell culture supernatant, Urine, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.04 ng/ml
    • Range

      0.08 ng/ml - 5 ng/ml
    • Recovery

      98 %

      Sample specific recovery
      Sample type Average % Range
      Serum 98 89% - 108%
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Human Superoxide Dismutase 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cu/Zn Superoxide Dismutase concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, fetal umbilical vein blood and urine.


      Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.

    • Notes

      ab119520 Human Superoxide Dismutase 1 ELISA kit has been shown to detect both SOD1 and SOD3.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Assay Buffer Concentrate 1 x 5ml
      20X Wash Buffer Concentrate 1 x 50ml
      Adhesive Films 2 units
      HRP-Conjugate anti-Human Cu/Zn Superoxide Dismutase monoclonal antibody 2 x 20µl
      Human Cu/Zn Superoxide Dismutase Standard (5 ng/mL) 2 x 500µl
      Microplate coated with anti Human Cu/Zn SOD monoclonal antibody (12 x 8 wells) 1 unit
      Phosphate Buffered Saline Concentrate (PBS) (20x) 1 x 5ml
      Stop Solution (1M Phosphoric acid) 1 x 15ml
      TMB Substrate Solution 1 x 15ml
    • Research areas

      • Cell Biology
      • Other Antibodies
      • Oxidative Stress
      • Cancer
      • Cancer Metabolism
      • Cellular metabolic process
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cancer proteins ELISA kits
      • Metabolism
      • Types of disease
      • Cancer
      • Neuroscience
      • Diseases
    • Cellular localization

      Cytoplasmic. Note: The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
    • Alternative names

      • ALS
      • ALS1
      • Amyotrophic Lateral Sclerosis 1 (Adult)
      • Cu,Zn Superoxide Dismutase, EC 1.15.1.111
      • Cu/Zn Superoxide Dismutase
      • Homodimer
      • HSod1
      • Indophenoloxidase A
      • IPOA
      • SOD
      • Superoxide Dismutase 1, Soluble
      • Superoxide Dismutase, Cystolic
      see all
    • Database links

      • Entrez Gene: 6647 Human
      • Omim: 147450 Human
      • SwissProt: P00441 Human

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab119520.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (5)

      Publishing research using ab119520? Please let us know so that we can cite the reference in this datasheet.

      ab119520 has been referenced in 5 publications.

      • Shokrzadeh M  et al. An engineered non-erythropoietic erythropoietin-derived peptide, ARA290, attenuates doxorubicin induced genotoxicity and oxidative stress. Toxicol In Vitro 66:104864 (2020). PubMed: 32335150
      • Cao Y  et al. PARP1 might enhance the therapeutic effect of tetrahydroxystilbene glucoside in traumatic brain injury via inhibition of Ras/JNK signalling pathway. Folia Neuropathol 58:45-56 (2020). PubMed: 32337957
      • Koziol K  et al. Changes in ?H2AX and H4K16ac levels are involved in the biochemical response to a competitive soccer match in adolescent players. Sci Rep 10:14481 (2020). PubMed: 32879387
      • Bai J  et al. Eriodictyol inhibits high glucose-induced extracellular matrix accumulation, oxidative stress, and inflammation in human glomerular mesangial cells. Phytother Res 33:2775-2782 (2019). PubMed: 31373419
      • Stradling JR  et al. Biomarkers of oxidative stress following continuous positive airway pressure withdrawal: data from two randomised trials. Eur Respir J 46:1065-71 (2015). Sandwich ELISA ; Human . PubMed: 26022961

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      Could this kit recognize the G93A mutant protein, mutated at residue 93?
      How should cell culture supernatants be prepared? There are specific instructions about how to prepare other sample types.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 20 2012

      Answer

      Thank you for your call last week and for your patience while I have been in touch with the lab about your enquiry.

      Theepitopse of the antibodies used in the kit have not been mapped at this point, and the kit has never been tested with samples containing the G93A mutant protein, so unfortunately we do not know for sure if the kit will work with these samples.

      For cell culture supernatants, there isn't a particular recommended dilution because the concentration of the analyte can vary so much between these samples. We suggest trying several dilutions in order to find the optimal dilution for your particular samples.

      I am sorry thatwe don't have further information, but please let me know if you have any other questions or if there is anything else that we can do for you, and I'll be happy to help.

      Read More

      Abcam Scientific Support

      Answered on Feb 20 2012

      Question

      Hello, I would like to find out if you would sell the antibody pair (un-conjugated) and protein standard for human Cu/Zn Superoxide Dismutase. I would like to purchase enough reagents for ~5 plates for a proof-of-concept expt.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 22 2011

      Answer

      Thank you for contacting us with your enquiry. I have looked through our catalogue and have found two sets that may be of interest to use. ab88404: https://www.abcam.com/Superoxide-Dismutase-1-antibody-ab88404.html and it's peptide ab99369: https://www.abcam.com/Superoxide-Dismutase-1-peptide-ab99369.html Another set that may interest you are: ab45777 : https://www.abcam.com/Superoxide-Dismutase-1-antibody-ab45777.html the related peptide is ab46993: https://www.abcam.com/Superoxide-Dismutase-1-peptide-ab46993.html We also offer a Superoxide Dismutase Activity Colorimetric Assay Kit (ab65354) and a Cu/Zn Superoxide Dismutase Human ELISA kit (ab119520). I hope this information is helpful. Please contact us if you have any further questions.

      Read More

      Abcam Scientific Support

      Answered on Dec 22 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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