Human Surfactant protein D / SP-D ELISA Kit (ab213827)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 625 pg/ml - 40000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Surfactant protein D / SP-D ELISA Kit
See all Surfactant protein D/SP-D kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 1.547ng/ml 0.092 = 6% 2 16 3.58ng/ml 0.221 = 6.2% 3 16 25.269ng/ml 1.693 = 6.7% Inter-assay Sample n Mean SD CV% 1 24 1.591ng/ml 0.111 = 7% 2 24 3.588ng/ml 0.279 = 7.8% 3 24 23.717ng/ml 1.683 = 7.1% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
625 pg/ml - 40000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Surfactant protein D / SP-D Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213827) is designed for the quantitative measurement of Human Surfactant protein D / SP-D in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for Surfactant protein D / SP-D has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A21-F375) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Surfactant protein D / SP-D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Surfactant protein D / SP-D amount of sample captured in plate.
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Notes
Surfactant, pulmonary-associated protein D, also known as SFTPD / SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the Surfactant protein D / SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for Surfactant protein D / SP-D include roles in innate immunity and surfactant metabolism. Surfactant protein D / SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and Surfactant protein D / SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Surfactant protein D / SP-D coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human Surfactant protein D / SP-D antibody 1 x 100µl Lyophilized recombinant Human Surfactant protein D / SP-D standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. -
Sequence similarities
Belongs to the SFTPD family.
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsThe N-terminus is blocked.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film. - Information by UniProt
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Alternative names
- COLEC 7
- COLEC7
- Collectin-7
see all -
Database links
- Entrez Gene: 6441 Human
- Omim: 178635 Human
- SwissProt: P35247 Human
- Unigene: 253495 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213827 has not yet been referenced specifically in any publications.