Overview

  • Product name

    Human Survivin Matched Antibody Pair Kit
    See all Survivin kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    2 pg/ml - 125 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human survivin.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab183361) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


     


     

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human Survivin Capture Antibody 1 x 100µg 1 x 20µg
    Human Survivin Detector Antibody 1 x 25µg 1 x 5µg
    Human Survivin Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • Tissue specificity

    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Sequence similarities

    Belongs to the IAP family.
    Contains 1 BIR repeat.
  • Developmental stage

    Expression is cell cycle-dependent and peaks at mitosis.
  • Domain

    The BIR repeat is necessary and sufficient for HBXIP binding.
  • Post-translational
    modifications

    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • Alternative names

    • API4
    • Apoptosis inhibitor 4
    • Apoptosis inhibitor survivin
    • Apoptosis inhibitor4
    • Baculoviral IAP repeat containing 5
    • Baculoviral IAP repeat containing protein 5
    • Baculoviral IAP repeat-containing protein 5
    • BIRC 5
    • BIRC5
    • BIRC5_HUMAN
    • EPR 1
    • IAP4
    • Survivin variant 3 alpha
    • SVV
    • TIAP
    see all
  • Database links

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab222257 has not yet been referenced specifically in any publications.

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