Human Syndecan-1 ELISA Set (without plates) (ab47352)
- Datasheet
- References
- Protocols
Overview
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Product name
Human Syndecan-1 ELISA Set (without plates)
See all Syndecan-1 kits -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 2.56 ng/ml -
Range
8 ng/ml - 256 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Syndecan-1 Human in vitro ELISA Set is designed for the quantitative measurement of Syndecan-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human Syndecan-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The Syndecan-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human Syndecan-1.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-Syndecan-1 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials Syndecan-1 Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml Syndecan-1 Standard (lyophilized) Yellow 5 vials 10 vials 15 vials -
Research areas
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Function
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. -
Sequence similarities
Belongs to the syndecan proteoglycan family. -
Post-translational
modificationsShedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. -
Cellular localization
Membrane. Secreted. Shedding of the ectodomain produces a soluble form. - Information by UniProt
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Alternative names
- CD138
- CD138 antigen
- SDC
see all -
Database links
- Entrez Gene: 6382 Human
- SwissProt: P18827 Human
- Unigene: 224607 Human
- Unigene: 665958 Human
Applications
Our Abpromise guarantee covers the use of ab47352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Syndecan Human ELISA Set (ab47352)Syndecans standard curve ranging from 8 to 256 ng/ml
Protocols
References
ab47352 has not yet been referenced specifically in any publications.
