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Human Syndecan-1 ELISA Set (without plates) (ab47352)

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Syndecan Human ELISA Set (ab47352)

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    Overview

    • Product name

      Human Syndecan-1 ELISA Set (without plates)
      See all Syndecan-1 kits
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 2.56 ng/ml
    • Range

      8 ng/ml - 256 ng/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Syndecan-1 Human in vitro ELISA Set is designed for the quantitative measurement of Syndecan-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

       

      This ELISA Set is intended to measure human Syndecan-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

       

      The Syndecan-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

       

      Recognizes both natural and recombinant human Syndecan-1.

    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests
      Detection biotinylated anti-Syndecan-1 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
      Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
      Streptavidin-HRP 1 vial 2 vials 3 vials
      Syndecan-1 Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
      Syndecan-1 Standard (lyophilized) Yellow 5 vials 10 vials 15 vials
    • Research areas

      • Neuroscience
      • Cell Adhesion Proteins
      • Membrane Proteins
      • Neuroscience
      • Cell Adhesion Proteins
      • ECM Proteins
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Proteins
      • Glucosaminoglycans
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Signal Transduction
      • Cytoskeleton / ECM
      • HSPGs
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Cell adhesion
      • Other
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • Other
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Adhesion molecules ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELIPAIR Kit and ELISA Set
      • Adhesion molecules ELIPAIR kits
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
    • Function

      Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
    • Sequence similarities

      Belongs to the syndecan proteoglycan family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P18827 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • CD_antigen
      • CD138
      • CD138 antigen
      • heparan sulfate proteoglycan fibroblast growth factor receptor
      • SDC
      • Sdc1
      • SDC1_HUMAN
      • SYND1
      • Syndecan
      • Syndecan 1
      • syndecan proteoglycan 1
      • Syndecan-1
      see all
    • Database links

      • Entrez Gene: 6382 Human
      • Omim: 186355 Human
      • SwissProt: P18827 Human
      • Unigene: 224607 Human
      • Unigene: 665958 Human

      Associated products

        Applications

        The Abpromise guarantee

        Our Abpromise guarantee covers the use of ab47352 in the following tested applications.

        The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

        Application Abreviews Notes
        Sandwich ELISA
        Use at an assay dependent concentration.
        Notes
        Sandwich ELISA
        Use at an assay dependent concentration.

        Images

        • Syndecan Human ELISA Set (ab47352)
          Syndecan Human ELISA Set (ab47352)

          Syndecans standard curve ranging from 8 to 256 ng/ml

        Protocols

        • Human Syndecan Elipair
        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • SDS download

        • Datasheet download

          Download

        References (1)

        Publishing research using ab47352? Please let us know so that we can cite the reference in this datasheet.

        ab47352 has been referenced in 1 publication.

        • Orecchia P  et al. L19-IL2 Immunocytokine in Combination with the Anti-Syndecan-1 46F2SIP Antibody Format: A New Targeted Treatment Approach in an Ovarian Carcinoma Model. Cancers (Basel) 11:N/A (2019). PubMed: 31443604

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