Human Syndecan-1 ELISA Set (without plates) (ab47352)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameHuman Syndecan-1 ELISA Set (without plates)
See all Syndecan-1 kits -
Sample typeCell culture supernatant, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 2.56 ng/ml
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Range8 ng/ml - 256 ng/ml
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s Syndecan-1 Human in vitro ELISA Set is designed for the quantitative measurement of Syndecan-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human Syndecan-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The Syndecan-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human Syndecan-1.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformReagents
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-Syndecan-1 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials Syndecan-1 Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml Syndecan-1 Standard (lyophilized) Yellow 5 vials 10 vials 15 vials -
Research areas
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FunctionCell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
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Sequence similaritiesBelongs to the syndecan proteoglycan family.
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Post-translational
modificationsShedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. -
Cellular localizationMembrane. Secreted. Shedding of the ectodomain produces a soluble form.
- Information by UniProt
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Alternative names
- CD138
- CD138 antigen
- SDC
see all -
Database links
- Entrez Gene: 6382 Human
- SwissProt: P18827 Human
- Unigene: 224607 Human
- Unigene: 665958 Human
Applications
Our Abpromise guarantee covers the use of ab47352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Syndecan Human ELISA Set (ab47352)Syndecans standard curve ranging from 8 to 256 ng/ml
Protocols
References
ab47352 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"