Overview

  • Product name

    Human Syndecan-4 ELISA Kit (SDC4)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 630pg/ml 26.5 4.2%
    2 16 1447pg/ml 83.9 5.8%
    3 16 2135pg/ml 100.3 4.7%
    Inter-assay
    Sample n Mean SD CV%
    1 24 718pg/ml 35.2 4.9%
    2 24 1604pg/ml 104.3 6.5%
    3 24 2369pg/ml 120.8 5.1%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Syndecan-4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (SDC4) (ab213830) is designed for the quantitative measurement of Human Syndecan-4 in cell culture supernatants and serum.


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Syndecan-4/SDC4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E19-E145) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Syndecan-4/SDC4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Syndecan-4/SDC4 amount of sample captured in plate.

  • Notes

    Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecan’s and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Syndecan-4 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human Syndecan-4 antibody 1 x 130µl
    Lyophilized recombinant Human Syndecan-4 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Cell surface proteoglycan that bears heparan sulfate.
  • Tissue specificity

    Expressed in epithelial and fibroblastic cells.
  • Sequence similarities

    Belongs to the syndecan proteoglycan family.
  • Post-translational
    modifications

    Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
  • Cellular localization

    Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
  • Information by UniProt
  • Alternative names

    • Amphiglycan
    • MGC22217
    • OTTHUMP00000031788
    • Ryudocan
    • ryudocan amphiglycan
    • Ryudocan core protein
    • SDC 4
    • Sdc4
    • SDC4_HUMAN
    • SYND 4
    • SYND4
    • syndecan 4
    • syndecan 4 (amphiglycan, ryudocan)
    • syndecan proteoglycan 4
    • Syndecan-4
    • Syndecan4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Syndecan-4 ELISA Kit (SDC4) (ab213830) Standard Curve.

Protocols

References

ab213830 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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