Key features and details
- Sensitivity: 70 pg/ml
- Range: 78.15 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman TACE ELISA Kit (ADAM17)
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 70 pg/ml
Range78.15 pg/ml - 5000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.65 89% - 124% Serum 112.3 85% - 132% Plasma 101.1 72% - 128%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s TACE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TACE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
The standard is affinity purified.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human TACE Item F 2 vials Recombinant Human TACE Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TACE Microplate (12 x 8 wells) Item A 1 unit TMB One-Step Substrate Reagent 1 x 12ml
- SwissProt: P78536 Human
ab113321 has been referenced in 1 publication.
- Erdoes G et al. CD62L (L-selectin) shedding for assessment of perioperative immune sensitivity in patients undergoing cardiac surgery with cardiopulmonary bypass. PLoS One 8:e53045 (2013). ELISA ; Human . PubMed: 23301018