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  1. Link

    human-taok3-kds-knockout-hela-cell-lysate-ab258222.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Human TAOK3 (KDS) knockout HeLa cell lysate (ab258222)

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Western blot - Human TAOK3 (KDS) knockout HeLa cell lysate (ab258222)
  • Sanger Sequencing - Human TAOK3 knockout HeLa cell lysate (ab258222)
  • Sanger Sequencing - Human TAOK3 knockout HeLa cell lysate (ab258222)

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Protein
Product image
Recombinant human KDS protein (ab125551)
Primary
Product image
Anti-KDS antibody [EPR4947(2)] (ab150388)
Knockout
Product image
Human TAOK3 (KDS) knockout HeLa cell line (ab265108)

View more associated products

Overview

  • Product name

    Human TAOK3 (KDS) knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 146 bp insertion in exon6 and Insertion of the selection cassette in exon6.
  • Passage number

    <20
  • Knockout validation

    Sanger Sequencing, Western Blot (WB)
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261179 - Human TAOK3 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Target

  • Function

    Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.
  • Tissue specificity

    Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Cell membrane. Also localized to the peripheral cell membrane.
  • Target information above from: UniProt accession Q9H2K8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CTCL tumor antigen HD CL 09
    • CTCL-associated antigen HD-CL-09
    • Cutaneous T cell lymphoma tumor antigen HD CL 09
    • Cutaneous T-cell lymphoma-associated antigen HD-CL-09
    • Dendritic cell derived protein kinase
    • Dendritic cell-derived protein kinase
    • DKFZp666H245
    • DPK
    • FLJ31808
    • hKFC A
    • hKFC-A
    • JIK
    • JNK/SAPK inhibitory kinase
    • JNK/SAPK-inhibitory kinase
    • Jun kinase inhibitory kinase
    • Jun kinase-inhibitory kinase
    • KDS
    • Kinase from chicken homolog A
    • MAP3K18
    • Serine kinase
    • Serine/threonine-protein kinase TAO3
    • STE20 like kinase
    • TAO kinase 3
    • TAOK3
    • TAOK3_HUMAN
    • Thousand and one amino acid protein 3
    see all

Associated products

  • KO cell lines

    • Human TAOK3 (KDS) knockout HeLa cell line (ab265108)
  • Related Products

    • Anti-KDS antibody [EPR4947(2)] (ab150388)
    • Anti-KDS antibody [EPR4947(2)] - BSA and Azide free (ab248958)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab258222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 105 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 105 kDa.

Images

  • Western blot - Human TAOK3 (KDS) knockout HeLa cell lysate (ab258222)
    Western blot - Human TAOK3 (KDS) knockout HeLa cell lysate (ab258222)
    Lane 1: Wild-type HeLa cell lysate (40µg)

    Lane 2: TAOK3 knockout HeLa cell lysate (40µg)

    Lane 3: Jurkat cell lysate (10µg)

    Lane 4: Daudi cell lysate (10µg)

    Lanes 1- 4: Merged signal (red and green). Green - ab150388 observed at 105 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab150388 Rabbit monoclonal [EPR4947(2)] to KDS was shown to specifically react with KDS in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265108 (knockout cell lysate ab258222) was used. Wild-type and KDS knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab150388 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Sanger Sequencing - Human TAOK3 knockout HeLa cell lysate (ab258222)
    Sanger Sequencing - Human TAOK3 knockout HeLa cell lysate (ab258222)

    Allele-1: 146 bp insertion in exon6

     

  • Sanger Sequencing - Human TAOK3 knockout HeLa cell lysate (ab258222)
    Sanger Sequencing - Human TAOK3 knockout HeLa cell lysate (ab258222)

    Allele-2: Insertion of the selection cassette in exon6

     

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab258222? Please let us know so that we can cite the reference in this datasheet.

ab258222 has not yet been referenced specifically in any publications.

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