• Product name

    Human TARC ELISA Kit
    See all TARC kits
  • Detection method

  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 5 pg/ml
  • Range

    4.1 pg/ml - 1000 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106.7 98% - 122%
    Serum 76.75 70% - 93%
    Plasma 78.32 73% - 97%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s TARC Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TARC in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for Human TARC coated on a 96-well plate. Standards and samples are pipetted into the wells and TARC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TARC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TARC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Get results in 90 minutes with Human TARC ELISA Kit (CCL17) (ab183366) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    Assay Diluent C 1 x 30ml
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Biotinylated anti-Human TARC (lyophilized) 2 vials
    Recombinant Human TARC Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TARC Microplate (12 x 8 wells) 1 unit
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4.
  • Tissue specificity

    Expressed at high levels in thymus and at low levels in the lung, colon and small intestine.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

  • Information by UniProt
  • Alternative names

    • A 152E5.3
    • ABCD 2
    • C C motif chemokine 17
    • C-C motif chemokine 17
    • CC chemokine TARC
    • CCL 17
    • CCL17
    • CCL17_HUMAN
    • Chemokine (C C motif) ligand 17
    • Chemokine ligand 17
    • MGC138271
    • MGC138273
    • SCYA17
    • Small inducible cytokine A17
    • Small inducible cytokine subfamily A (Cys Cys) member 17
    • Small inducible cytokine subfamily A member 17
    • Small-inducible cytokine A17
    • T cell directed CC chemokine
    • Thymus and activation regulated chemokine
    • Thymus and activation-regulated chemokine
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab100644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100644



ab100644 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

CCL17 secretion by human macrophages

Excellent Excellent 5/5 (Ease of Use)
CCL17 secretion by human macrophages isolated by negative selection in culture for 3 and 7 days.
Kit easy to use, the assay takes about 5.30hr, excellent specificity as shown by higher secretion by M2a macrophages (MSCF+IL4) on day 7 compared to the others subtypes (M0=MCSF M1=MSCF+IFNg DEX=MCSF+Dexamethason IL10=MCSF+IL10 TGFb=MCSF+TGFb)

Abcam user community

Verified customer

Submitted Apr 01 2014


Thanks for your inquiry. Based upon sequence homology, I would not expect any of the antibodies to cross react with mouse as there is only a 64% homology. Please let me know if you require further assistance.

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