Human TARC ELISA Kit (ab100644)
- Datasheet
- References
- Protocols
Overview
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Product name
Human TARC ELISA Kit
See all TARC kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 5 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
87 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 106.7 98% - 122% Serum 76.75 70% - 93% Plasma 78.32 73% - 97% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s TARC Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TARC in serum, plasma and cell culture supernates.
This assay employs an antibody specific for Human TARC coated on a 96-well plate. Standards and samples are pipetted into the wells and TARC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TARC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TARC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human TARC ELISA Kit (CCL17) (ab183366) from our SimpleStep ELISA® range.
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Notes
Optimization may be required with urine samples.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests Assay Diluent C 1 x 30ml 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Biotinylated anti-Human TARC (lyophilized) 2 vials Recombinant Human TARC Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TARC Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. -
Tissue specificity
Expressed at high levels in thymus and at low levels in the lung, colon and small intestine. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A 152E5.3
- ABCD 2
- C C motif chemokine 17
see all -
Database links
- Entrez Gene: 6361 Human
- Omim: 601520 Human
- SwissProt: Q92583 Human
- Unigene: 546294 Human
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab100644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical standard curveRepresentative standard curve using ab100644
Protocols
References
ab100644 has not yet been referenced specifically in any publications.
